| Identification of Novel Cyclic Nucleotide Phosphodiesterase Gene cDNAs in the Cochlea of Guinea Pig (Cavia porcellus) Through Conserved Homologous Sequences. | |
| | |
MedLine Citation:
|
PMID: 19707892 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
The nitric oxide (NO) signaling pathway plays a critical role in auditory signal conversion and transduction. Cyclic nucleotide phosphodiesterase (PDE), an important component of the NO signaling pathway, has not been identified in the cochlea. Using cross-species comparison, homologous sequences of human and mouse Pde coding sequences were searched in a guinea pig genomic database and conserved homologous exons were found between human and mouse homologous sequences. Based on reverse-transcription PCR of these conserved regions, six partial Pde cDNAs were detected in the cochlea: CpPde3a, CpPde4d, CpPde8a, CpPde8b, CpPde9a, and CpPde11a. The identity rates of the six partial Pde cDNA sequences between guinea pig and human range from 83.8 to 95.5% and those of the peptide sequences range from 85.6 to 100%. The identity rates of the six Pde cDNA sequences between guinea pig and mouse range from 80.6 to 93.0% and those of peptide sequences range from 79.5 to 99.2%. The results demonstrate that multiple Pde genes are expressed in the cochlea, suggesting a NO pathway in the auditory system. Insights into this pathway will help to develop new therapeutic drugs on auditory abnormalities. |
| | |
Authors:
|
Min-Qi Huang; Jian-Guo Zhang; Yue-Sheng Long |
Publication Detail:
|
Type: Journal Article Date: 2009-08-26 |
Journal Detail:
|
Title: Molecular biotechnology Volume: 44 ISSN: 1559-0305 ISO Abbreviation: Mol. Biotechnol. Publication Date: 2010 Jan |
Date Detail:
|
Created Date: 2009-12-18 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9423533 Medline TA: Mol Biotechnol Country: United States |
Other Details:
|
Languages: eng Pagination: 66-70 Citation Subset: IM |
Affiliation:
|
Institute of Neuroscience, The Second Affiliated Hospital, Guangzhou Medical College, 250 Chang-gang-dong Road, 510260, Guangzhou, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Epigenetics in Rheumatoid Arthritis.
Next Document: Redistribution pattern of fetal liver circulation in intrauterine growth restriction.