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Identification of Novel Cyclic Nucleotide Phosphodiesterase Gene cDNAs in the Cochlea of Guinea Pig (Cavia porcellus) Through Conserved Homologous Sequences.
MedLine Citation:
PMID:  19707892     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The nitric oxide (NO) signaling pathway plays a critical role in auditory signal conversion and transduction. Cyclic nucleotide phosphodiesterase (PDE), an important component of the NO signaling pathway, has not been identified in the cochlea. Using cross-species comparison, homologous sequences of human and mouse Pde coding sequences were searched in a guinea pig genomic database and conserved homologous exons were found between human and mouse homologous sequences. Based on reverse-transcription PCR of these conserved regions, six partial Pde cDNAs were detected in the cochlea: CpPde3a, CpPde4d, CpPde8a, CpPde8b, CpPde9a, and CpPde11a. The identity rates of the six partial Pde cDNA sequences between guinea pig and human range from 83.8 to 95.5% and those of the peptide sequences range from 85.6 to 100%. The identity rates of the six Pde cDNA sequences between guinea pig and mouse range from 80.6 to 93.0% and those of peptide sequences range from 79.5 to 99.2%. The results demonstrate that multiple Pde genes are expressed in the cochlea, suggesting a NO pathway in the auditory system. Insights into this pathway will help to develop new therapeutic drugs on auditory abnormalities.
Authors:
Min-Qi Huang; Jian-Guo Zhang; Yue-Sheng Long
Publication Detail:
Type:  Journal Article     Date:  2009-08-26
Journal Detail:
Title:  Molecular biotechnology     Volume:  44     ISSN:  1559-0305     ISO Abbreviation:  Mol. Biotechnol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423533     Medline TA:  Mol Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  66-70     Citation Subset:  IM    
Affiliation:
Institute of Neuroscience, The Second Affiliated Hospital, Guangzhou Medical College, 250 Chang-gang-dong Road, 510260, Guangzhou, China.
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