| Identification of Meiothermus as the dominant genus in a storage system for spent nuclear fuel. | |
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MedLine Citation:
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PMID: 15715877 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To characterize the biofilms in an interim wet storage system (CLAB) for spent nuclear fuel. METHODS AND RESULTS: Planktonic cells and biofilms were analysed with fluorescence microscopy, and scanning and transmission electron microscopy. The organisms in the biofilms were filamentous, consisting of sheaths with series of rod-shaped cells in them. Planktonic cell populations ranged between 1.4 x 10(3) and 5.2 x 10(3) ml(-1), correlated with the system configuration, and was inversely correlated with total organic carbon (TOC) levels. Analysis of 16S rDNA indicated that a potentially novel Meiothermus sp. was dominant in the CLAB biofilms. A Meiothermus-specific probe was designed, and statistical analysis of fluorescence in situ hybridization results confirmed that Meiothermus sp. composed up to 98% of the biofilm. The low TOC levels (2-55 microg l(-1)) in the system combined with elevated water temperatures (ca 36 degrees C) mimic the natural environments of Meiothermus sp. as well as the recommended conditions for isolating Meiothermus sp., thus supporting the results of the 16S rDNA analysis. CONCLUSIONS: A possibly novel Meiothermus sp. is the predominant genus found in the CLAB biofilms. SIGNIFICANCE AND IMPACT OF THE STUDY: TOC levels are often kept low to protect industrial systems from biofouling. However, the present study shows that, rather than protecting industrial systems against biofouling per se, maintaining low TOC levels might still result in biofilms, dominated by new, unknown bacterial species. |
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Authors:
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P Masurat; E C Fru; K Pedersen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of applied microbiology Volume: 98 ISSN: 1364-5072 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2005 |
Date Detail:
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Created Date: 2005-02-17 Completed Date: 2005-06-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 727-40 Citation Subset: IM |
Affiliation:
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Department of Cell and Molecular Biology, Göteborg University, Göteborg, Sweden. peter.masurat@gmm.gu.se |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biofilms Industrial Microbiology* Microscopy, Electron, Transmission Microscopy, Fluorescence Plankton RNA, Ribosomal, 16S / analysis Radioactive Waste* Temperature Thermus / genetics, isolation & purification* Waste Management* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Ribosomal, 16S; 0/Radioactive Waste |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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