| Identification of MSA1, a cell cycle-regulated, dosage suppressor of drc1/sld2 and dpb11 mutants. | |
| | |
MedLine Citation:
|
PMID: 18948746 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The Dpb11 and Drc1/Sld2 proteins form a complex that is critical for the initiation of DNA replication. In this study we identify MSA1 as a high copy suppressor of a drc1-1 mutant. MSA1 overproduction can also suppress the temperature sensitivity of dpb11-1 and pol2-12 mutants. Reciprocally, msa1 deletion exacerbates the mutant phenotypes of both drc1/sld2 and dpb11 mutants and msa1 deletion alone results in a delay in S phase entry of synchronous cells indicating a positive role for MSA1 in DNA replication. Paradoxically, MSA1 overproduction is deleterious to cdc6-1, cdc7-1, cdc28-1N and cdc14-1 mutants indicating a complex relationship with DNA replication and cell cycle regulatory genes. The Msa1 protein is tightly cell cycle regulated. Msa1 and its paralog, Msa2, both accumulate in highly modified forms just as cells commit to enter S phase and then are rapidly destroyed. MSA1 represents a new cell cycle regulated gene important for S phase entry. |
| | |
Authors:
|
Ju-mei Li; Michael T Tetzlaff; Stephen J Elledge |
Related Documents
:
|
17820116 - Scenedesmus obliquus sexuality. 6825116 - Persistence of ethyl carbamate-induced dna damage in vivo as indicated by sister chroma... 1692786 - Flow cytometric recognition of clastogen induced chromatin damage in g0/g1 lymphocytes ... 10878826 - Journey to the centre of the cell. 16966816 - Control of the bioluminescence starting time by inoculated cell density. 19832986 - Transcriptome analysis of functional differentiation between haploid and diploid cells ... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-11-05 |
Journal Detail:
|
Title: Cell cycle (Georgetown, Tex.) Volume: 7 ISSN: 1551-4005 ISO Abbreviation: Cell Cycle Publication Date: 2008 Nov |
Date Detail:
|
Created Date: 2008-12-23 Completed Date: 2009-02-10 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101137841 Medline TA: Cell Cycle Country: United States |
Other Details:
|
Languages: eng Pagination: 3388-98 Citation Subset: IM |
Affiliation:
|
Department of Genetics, Center for Genetics and Genomics, Brigham and Women's Hospital, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Cycle Cell Cycle Proteins / metabolism Gene Dosage* Genes, Suppressor* Genome, Fungal Mutation / genetics* Oligonucleotide Array Sequence Analysis Protein Binding S Phase Saccharomyces cerevisiae / cytology*, genetics* Saccharomyces cerevisiae Proteins / metabolism* Suppression, Genetic Temperature |
| Chemical | |
Reg. No./Substance:
|
0/Cell Cycle Proteins; 0/DPB11 protein, S cerevisiae; 0/Msa1 protein, S cerevisiae; 0/SLD2 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Arf-induced turnover of the nucleolar nucleophosmin-associated SUMO-2/3 protease Senp3.
Next Document: Transforming human blood stem and progenitor cells: a new way forward in leukemia modeling.