| Identification Keratin 1 as a cDDP-resistant protein in nasopharyngeal carcinoma cell lines. | |
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MedLine Citation:
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PMID: 22348822 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Multidrug resistance (MDR) to anticancer drugs is a major obstacle to successful chemotherapy of tumors. Understanding the molecular basis to chemoresistance is likely to provide better treatment. Cell lines resistant to cis-diamminedichloroplatinum (CNE2/cDDP) were established from human nasopharyngeal carcinoma (NPC) cell lines CNE2. Comparative proteomics involving 2-dimensional gel electrophoresis (2-DE) and ESI-Q-TOF-MS were performed on protein extracted from CNE2 and CNE2/cDDP cell lines to screen drug resistance-related proteins. Keratin 1 (KRT1), cathepsin D (CTSD) and annexin a5 (ANXA5) were identified as three proteins showing higher expression in CNE2/cDDP compared to CNE2. Furthermore, suppression of KRT1 expression by siRNA resulted in decreased MDR in siRNA-CNE2/cDDP cells. And upregulation of KRT1 could result in increased of drug resistance in NPC cell lines. Taken together, KRT1 protein and its activity levels were higher in cDDP-resistant NPC cell lines compared to their parental cell lines. These data clearly linked KRT1 and cDDP resistance mechanisms. KRT1 could serve as a biomarker for chemotherapy sensitivity of NPC. |
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Authors:
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Sanyuan Tang; Weiguo Huang; Meizuo Zhong; Leilan Yin; Hao Jiang; Suping Hou; Pingping Gan; Yujuan Yuan |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-2-12 |
Journal Detail:
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Title: Journal of proteomics Volume: - ISSN: 1876-7737 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-3-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101475056 Medline TA: J Proteomics Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Crown Copyright © 2012. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Oncology, Xiangya Hospital, Central South University, Changsha 410008, P. R. China; Oncology Medical Department of the First Affiliated Hospital, University of South China, Hengyang 421001, P. R. China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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