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Identification Keratin 1 as a cDDP-resistant protein in nasopharyngeal carcinoma cell lines.
MedLine Citation:
PMID:  22348822     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Multidrug resistance (MDR) to anticancer drugs is a major obstacle to successful chemotherapy of tumors. Understanding the molecular basis to chemoresistance is likely to provide better treatment. Cell lines resistant to cis-diamminedichloroplatinum (CNE2/cDDP) were established from human nasopharyngeal carcinoma (NPC) cell lines CNE2. Comparative proteomics involving 2-dimensional gel electrophoresis (2-DE) and ESI-Q-TOF-MS were performed on protein extracted from CNE2 and CNE2/cDDP cell lines to screen drug resistance-related proteins. Keratin 1 (KRT1), cathepsin D (CTSD) and annexin a5 (ANXA5) were identified as three proteins showing higher expression in CNE2/cDDP compared to CNE2. Furthermore, suppression of KRT1 expression by siRNA resulted in decreased MDR in siRNA-CNE2/cDDP cells. And upregulation of KRT1 could result in increased of drug resistance in NPC cell lines. Taken together, KRT1 protein and its activity levels were higher in cDDP-resistant NPC cell lines compared to their parental cell lines. These data clearly linked KRT1 and cDDP resistance mechanisms. KRT1 could serve as a biomarker for chemotherapy sensitivity of NPC.
Authors:
Sanyuan Tang; Weiguo Huang; Meizuo Zhong; Leilan Yin; Hao Jiang; Suping Hou; Pingping Gan; Yujuan Yuan
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-12
Journal Detail:
Title:  Journal of proteomics     Volume:  -     ISSN:  1876-7737     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-3-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101475056     Medline TA:  J Proteomics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Crown Copyright © 2012. Published by Elsevier B.V. All rights reserved.
Affiliation:
Department of Oncology, Xiangya Hospital, Central South University, Changsha 410008, P. R. China; Oncology Medical Department of the First Affiliated Hospital, University of South China, Hengyang 421001, P. R. China.
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