Document Detail

Identification of the IL-6-responsive element in an acute-phase-responsive human pancreatic secretory trypsin inhibitor-encoding gene.
MedLine Citation:
PMID:  7691687     Owner:  NLM     Status:  MEDLINE    
Pancreatic secretory trypsin inhibitor (PSTI) has been suggested to be an acute-phase reactant in humans and to be induced by inflammatory cytokines such as the interleukins IL-1 and IL-6. We report that PSTI is synthesized in hepatoma cells and that the gene expression is augmented by IL-6. The start points (tsp) for basal and augmented transcription are exactly the same as the tsp in normal pancreas. Analysis of the PSTI gene revealed that a 40-bp DNA fragment located between kb -3.84 and -3.80 carries the element responsible for both transcriptional activity and IL-6-induced gene expression. This 40-bp fragment contains TTGNNGNAATG, the consensus sequence for the NF-IL6-binding site, which is also known as the IL-6-responsive element that is conserved among various acute-phase genes. The basal activity was augmented by another sequence that lies between kb -4.0 and -3.9.
T Yasuda; M Ogawa; A Murata; Y Ohmachi; T Yasuda; T Mori; K Matsubara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Gene     Volume:  131     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-11-04     Completed Date:  1993-11-04     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  275-80     Citation Subset:  IM    
Department of Surgery II, Osaka University Medical School, Japan.
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MeSH Terms
Acute-Phase Proteins / genetics*
Base Sequence
Carcinoma, Hepatocellular
Consensus Sequence
Interleukin-6 / metabolism*
Liver / metabolism
Molecular Sequence Data
Pancreas / metabolism
RNA, Messenger / metabolism
Regulatory Sequences, Nucleic Acid*
Transcription, Genetic
Trypsin Inhibitor, Kazal Pancreatic / genetics*
Tumor Cells, Cultured
Reg. No./Substance:
0/Acute-Phase Proteins; 0/Interleukin-6; 0/RNA, Messenger; 50936-63-5/Trypsin Inhibitor, Kazal Pancreatic; 9007-49-2/DNA

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