| Identification of HepG2 variant cell lines by short tandem repeat (STR) analysis. | |
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MedLine Citation:
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PMID: 12619888 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the past years, in our laboratory, several cell lines have been generated starting from a human liver (H7). Some of them have been used successfully in studies of the infection with and propagation of Hepatitis B and Hepatitis C viruses. Recently, several lines of evidence indicated that the origin of these cell lines was uncertain. Therefore, we now have determined the genetic characteristics of these cell lines in comparison to HepG2 cells received from ATCC and to HepG2 isolates grown at other laboratories. Quadruplex fluorescent short tandem repeat (STR) typing and karyotyping were performed. In addition, some biochemical characteristics of selected clones were studied. Genetically, all H7-derived cell lines were identical to HepG2 cells. However, some liver-specific functions varied between the different sub-cloned lines. The H7-derived cell lines that were generated proved to be sub-cloned lines of HepG2. The problem of cross-contamination during cloning of cell lines appears to be not uncommon. We found that two out of six HepG2 isolates obtained from other laboratories were not derived from the same individual as the original HepG2 cells. Therefore, STR typing should be applied as a rapid and sensitive technique to determine and monitor the origin of cell lines and to safeguard against contamination. |
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Authors:
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Jos F van Pelt; Ronny Decorte; Paul S H Yap; Johan Fevery |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 243 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2003-03-06 Completed Date: 2003-11-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 49-54 Citation Subset: IM |
Affiliation:
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Department of Liver and Pancreatic Diseases, University Hospital Gasthuisberg, Leuven, Belgium. Jos.vanPelt@med.kuleuven.ac.be |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Albumins
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metabolism Bilirubin / metabolism Cell Line* Cell Line, Tumor* Culture Media / pharmacology DNA, Viral Fibrinogen / metabolism Hepatoblastoma / genetics, metabolism Hepatocytes / cytology*, metabolism Humans Karyotyping Lidocaine / pharmacokinetics Repetitive Sequences, Nucleic Acid* Simian virus 40 / genetics |
| Chemical | |
Reg. No./Substance:
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0/Albumins; 0/Culture Media; 0/DNA, Viral; 137-58-6/Lidocaine; 635-65-4/Bilirubin; 9001-32-5/Fibrinogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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