| Identification of Hb D-Punjab gene: application of DNA amplification in the study of abnormal hemoglobins. | |
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MedLine Citation:
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PMID: 2729278 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hemoglobin D-Punjab (or D-Los Angeles) is a common variant worldwide. It is also the most frequent abnormal hemoglobin in Xinjiang Uygur Autonomous Region of China. A large survey of hemoglobinopathy, including 142,171 people and 21 national/ethnic groups, was carried out in Xinjiang and indicated Hb D-Punjab accounted for 55.6% of the total hemoglobin variants there. Here we describe a simple way--EcoRI mapping of the amplified beta-globin DNA sampling from dried blood spots on filter paper blotters--of identifying the Hb D-Punjab gene. The primers were designed and synthesized to emzymatically amplify a 144-bp fragment of beta-globin gene which included codons beta 121 (GAA) and 122 (TTC) representing an EcoRI recognition site. The Hb D-Punjab gene could be easily detected by EcoRI digestion of the amplified DNA sequence on agarose gel because of a single base change at codon 121. The analysis of amplified DNA sampling from dried blood provides a very useful method for population study of Hb D-Punjab and will be of significance for demonstration of the occurrence of the Hb D-Punjab gene and for understanding of the relations among various nationalities. |
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Authors:
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Y T Zeng; S Z Huang; Z R Ren; H J Li |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of human genetics Volume: 44 ISSN: 0002-9297 ISO Abbreviation: Am. J. Hum. Genet. Publication Date: 1989 Jun |
Date Detail:
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Created Date: 1989-06-28 Completed Date: 1989-06-28 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0370475 Medline TA: Am J Hum Genet Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 886-9 Citation Subset: IM |
Affiliation:
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Laboratory of Medical Genetics, Shanghai Children's Hospital, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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China DNA / analysis DNA-Directed DNA Polymerase Gene Amplification* Hemoglobins, Abnormal / genetics* Humans Nucleic Acid Hybridization Restriction Mapping Taq Polymerase |
| Grant Support | |
ID/Acronym/Agency:
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HLB-29623/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Hemoglobins, Abnormal; 9007-49-2/DNA; 9034-60-0/hemoglobin D Punjab; EC 2.7.7.-/Taq Polymerase; EC 2.7.7.7/DNA-Directed DNA Polymerase |
| Comments/Corrections | |
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