Document Detail


Identification of HN-1-Peptide Target in Head and Neck Squamous Cell Carcinoma Cells.
MedLine Citation:
PMID:  22084724     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The HN-1 module was previously reported to ensure efficient targeting of head and neck squamous cell carcinoma (HNSCC). Aim of this work was to indentify the target of HN-1. Targeting of HN-1 peptide was compared in normal epithelial cells (BEAS-2B) and in HNSCC tumor cells (SCC-25 and Detroit 562). Experimental, cell culture, cell polarity, and adhesion conditions were tested; structure models of peptides were created. Indeed, HN-1 was able to target HNSCC tumor cells in the previously published conditions. The targeting efficiency of immortalized normal epithelial cells was significantly lower. Nevertheless, in other experimental conditions the binding was less efficient and not specific. A scrambled sequence of HN-1, with altered order of amino acids showed even better targeting efficiency than HN-1. HN-1 was only uptaken in adherent cells, not in suspension. In conclusion, HN-1-peptide-targeting is not based on sequence specificity, but more on electrostatic interactions with the cell surface of the tumor cells.
Authors:
Jozsef Dudas; Christin Idler; Georg Sprinzl; Andreas Bernkop-Schnuerch; Herbert Riechelmann
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Publication Detail:
Type:  Journal Article     Date:  2011-05-03
Journal Detail:
Title:  ISRN oncology     Volume:  2011     ISSN:  2090-567X     ISO Abbreviation:  ISRN Oncol     Publication Date:  2011  
Date Detail:
Created Date:  2011-11-15     Completed Date:  2011-11-23     Revised Date:  2012-06-26    
Medline Journal Info:
Nlm Unique ID:  101567026     Medline TA:  ISRN Oncol     Country:  Egypt    
Other Details:
Languages:  eng     Pagination:  140316     Citation Subset:  -    
Affiliation:
Department of Otorhinolaryngology, Medical University Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria.
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MeSH Terms
Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
P 22287-B13//Austrian Science Fund FWF

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