| Identification of HLA-A*3101-restricted cytotoxic T-lymphocyte response to human immunodeficiency virus type 1 (HIV-1) in patients with chronic HIV-1 infection. | |
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MedLine Citation:
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PMID: 16185326 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Virus-specific cytotoxic T-lymphocyte (CTL) responses are critical in the control of human immunodeficiency virus type 1 (HIV-1) infections. As several HIV-1 CTL epitopes restricted to many HLA types are already known, we aimed at identifying the CTL epitopes restricted by HLA-A*3101 in an effort to expand the epitope repertoire available for the development of potential T cell-mediated therapeutic measures and protective vaccines. Scanning of HIV-1 clade B SF2 strain proteins for the presence of peptides containing HLA-A*3101-binding motifs revealed 88 nine- to 11-mer peptides that had been synthesized and assayed for binding to HLA-A*3101 molecules. Peptides with medium to high HLA-binding affinity were tested for their ability to stimulate a CTL response in the peripheral blood mononuclear cells (PBMCs) from selected HIV-1-infected patients. Two of these binding peptides, Env769-779 (RLRDLLLIAAR) and Nef192-200 (KLAFHHMAR), induced peptide-specific CTLs in PBMCs from at least two of five HIV-1-seropositive individuals. CTL clones specific for the two peptides killed HLA-A*3101-expressing target cells infected with HIV-1 recombinant vaccinia virus, indicating that these peptides were naturally processed HLA-A*3101-restricted CTL epitopes. Identification of T-cell epitopes on HIV-1 proteins will increase our understanding of the role of CD8+ T cells in HIV-1 infections and assist in the design of new protective strategies. |
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Authors:
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M A Borghan; S Oka; M Takiguchi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Tissue antigens Volume: 66 ISSN: 0001-2815 ISO Abbreviation: Tissue Antigens Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-09-27 Completed Date: 2005-12-20 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0331072 Medline TA: Tissue Antigens Country: Denmark |
Other Details:
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Languages: eng Pagination: 305-13 Citation Subset: IM |
Affiliation:
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Division of Viral Immunology, Center for AIDS Research, Kumamoto University, Kumamoto, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Epitopes, T-Lymphocyte
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genetics,
immunology* Female Gene Products, env / immunology Gene Products, nef / genetics, immunology HIV Infections / genetics, immunology* HIV-1 / immunology* HLA-A Antigens / genetics, immunology* Humans Male Peptides / genetics, immunology T-Lymphocyte Subsets / immunology*, virology T-Lymphocytes, Cytotoxic / immunology*, virology nef Gene Products, Human Immunodeficiency Virus |
| Chemical | |
Reg. No./Substance:
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0/Epitopes, T-Lymphocyte; 0/Gene Products, env; 0/Gene Products, nef; 0/HLA-A Antigens; 0/HLA-A*3101 antigen, human; 0/Peptides; 0/nef Gene Products, Human Immunodeficiency Virus |
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