Document Detail

Identification of genes important for growth of asymptomatic bacteriuria Escherichia coli in urine.
MedLine Citation:
PMID:  22753377     Owner:  NLM     Status:  MEDLINE    
Escherichia coli is the most important etiological agent of urinary tract infections (UTIs). Unlike uropathogenic E. coli, which causes symptomatic infections, asymptomatic bacteriuria (ABU) E. coli strains typically lack essential virulence factors and colonize the bladder in the absence of symptoms. While ABU E. coli can persist in the bladder for long periods of time, little is known about the genetic determinants required for its growth and fitness in urine. To identify such genes, we have employed a transposon mutagenesis approach using the prototypic ABU E. coli strain 83972 and the clinical ABU E. coli strain VR89. Six genes involved in the biosynthesis of various amino acids and nucleobases were identified (carB, argE, argC, purA, metE, and ilvC), and site-specific mutants were subsequently constructed in E. coli 83972 and E. coli VR89 for each of these genes. In all cases, these mutants exhibited reduced growth rates and final cell densities in human urine. The growth defects could be complemented in trans as well as by supplementation with the appropriate amino acid or nucleobase. When assessed in vivo in a mouse model, E. coli 83972carAB and 83972argC showed a significantly reduced competitive advantage in the bladder and/or kidney during coinoculation experiments with the parent strain, whereas 83972metE and 83972ilvC did not. Taken together, our data have identified several biosynthesis pathways as new important fitness factors associated with the growth of ABU E. coli in human urine.
Rebecca M Vejborg; Mari R de Evgrafov; Minh Duy Phan; Makrina Totsika; Mark A Schembri; Viktoria Hancock
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-02
Journal Detail:
Title:  Infection and immunity     Volume:  80     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-15     Completed Date:  2012-10-24     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3179-88     Citation Subset:  IM    
Epidemiology and Microbial Genomics, DTU Food, Technical University of Denmark, Kongens Lyngby, Denmark.
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MeSH Terms
Bacteriuria / microbiology*
DNA Transposable Elements
Disease Models, Animal
Escherichia coli / genetics*,  growth & development*,  isolation & purification
Escherichia coli Infections / microbiology*
Gene Deletion
Gene Targeting
Genes, Bacterial*
Genetic Complementation Test
Human Experimentation
Mice, Inbred C3H
Mutagenesis, Insertional
Urine / microbiology*
Virulence Factors / genetics*
Reg. No./Substance:
0/DNA Transposable Elements; 0/Virulence Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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