Document Detail


Identification of differentially expressed genes between cloned and zygote-developing zebrafish (Danio rerio) embryos at the dome stage using suppression subtractive hybridization.
MedLine Citation:
PMID:  19092113     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Comparative analyses of differentially expressed genes between somatic cell nuclear transfer (SCNT) embryos and zygote-developing (ZD) embryos are important for understanding the molecular mechanism underlying the reprogramming processes. Herein, we used the suppression subtractive hybridization approach and from more than 2900 clones identified 96 differentially expressed genes between the SCNT and ZD embryos at the dome stage in zebrafish. We report the first database of differentially expressed genes in zebrafish SCNT embryos. Collectively, our findings demonstrate that zebrafish SCNT embryos undergo significant reprogramming processes during the dome stage. However, most differentially expressed genes are down-regulated in SCNT embryos, indicating failure of reprogramming. Based on Ensembl description and Gene Ontology Consortium annotation, the problems of reprogramming at the dome stage may occur during nuclear remodeling, translation initiation, and regulation of the cell cycle. The importance of regulation from recipient oocytes in cloning should not be underestimated in zebrafish.
Authors:
Daji Luo; Wei Hu; Shangping Chen; Yi Xiao; Yonghua Sun; Zuoyan Zhu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies     Date:  2008-12-17
Journal Detail:
Title:  Biology of reproduction     Volume:  80     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-24     Completed Date:  2009-07-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  674-84     Citation Subset:  IM    
Affiliation:
College of Life Sciences, Wuhan University, Wuhan, China.
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Animals
Cleavage Stage, Ovum / metabolism*
Cloning, Organism*
DNA / analysis
Embryo, Nonmammalian
Female
Gene Expression Profiling / methods
Gene Expression Regulation, Developmental*
Kidney / cytology,  embryology
Nuclear Transfer Techniques
Nucleic Acid Hybridization / methods
Oligonucleotide Array Sequence Analysis / methods
Zebrafish / embryology*,  genetics,  metabolism
Zygote / growth & development*,  metabolism
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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