| Identification of DELE, a novel DAP3-binding protein which is crucial for death receptor-mediated apoptosis induction. | |
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MedLine Citation:
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PMID: 20563667 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Death associated protein 3 (DAP3) is known to be a highly conserved protein, and is responsible for regulating apoptosis induced by various stimuli. To understand the molecular mechanism of how DAP3 induces apoptosis, we performed yeast two-hybrid screening, and identified a novel DAP3-binding protein termed death ligand signal enhancer (DELE). In this report, we show that DELE actually binds to DAP3 in mammalian cells. We found that the cells stably expressing DELE are susceptible to apoptosis induction by the stimulation of TNF-α and TRAIL. In addition, knockdown of DELE expression rescued the HeLa cells from apoptosis induction by these stimuli. Moreover, activation of caspase-3, caspase-8 and caspase-9 induced by stimulation of TNF-α, anti-Fas or TRAIL was significantly inhibited by the knockdown of DELE expression. These results demonstrated the biological significance of DELE for apoptosis signal mediated by death receptors. |
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Authors:
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Tanenobu Harada; Atsushi Iwai; Tadaaki Miyazaki |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Apoptosis : an international journal on programmed cell death Volume: 15 ISSN: 1573-675X ISO Abbreviation: Apoptosis Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-01 Completed Date: 2010-12-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9712129 Medline TA: Apoptosis Country: United States |
Other Details:
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Languages: eng Pagination: 1247-55 Citation Subset: IM |
Affiliation:
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Department of Bioresources, Hokkaido University Research Center for Zoonosis Control, North 20, West 10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* Apoptosis Regulatory Proteins / metabolism* Caspase 3 / metabolism Caspase 8 / metabolism Caspase 9 / metabolism Cell Line Gene Expression Gene Knockdown Techniques Hela Cells Humans Mitochondria / genetics, metabolism Proteins / genetics, metabolism* RNA, Small Interfering Receptors, Death Domain / metabolism* Ribosomal Proteins / metabolism* Signal Transduction TNF-Related Apoptosis-Inducing Ligand / metabolism Tumor Necrosis Factor-alpha / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Apoptosis Regulatory Proteins; 0/DAP3 protein, human; 0/DELE protein, human; 0/Proteins; 0/RNA, Small Interfering; 0/Receptors, Death Domain; 0/Ribosomal Proteins; 0/TNF-Related Apoptosis-Inducing Ligand; 0/Tumor Necrosis Factor-alpha; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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