Document Detail


Identification of D-aspartate in rat pheochromocytoma PC12 cells.
MedLine Citation:
PMID:  9665663     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 10(7) cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D-aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.
Authors:
Y Moriyama; H Yamada; M Hayashi; T Oda; A Yamaguchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  248     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1998 May 
Date Detail:
Created Date:  1999-03-30     Completed Date:  1999-03-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  57-60     Citation Subset:  IM    
Affiliation:
Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Japan. moriyama@sanken.osaka-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies
Aspartic Acid / analysis*,  immunology
Chromatography, High Pressure Liquid
Coloring Agents
Hela Cells
Humans
Immunohistochemistry
Isomerism
PC12 Cells / chemistry*
Rats
Chemical
Reg. No./Substance:
0/Antibodies; 0/Coloring Agents; 56-84-8/Aspartic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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