| Identification of cross-reactive norovirus CD4+ T cell epitopes. | |
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MedLine Citation:
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PMID: 20573810 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immune responses and the components of protective immunity following norovirus infection in humans are poorly understood. Although antibody responses following norovirus infection have been partially characterized, T cell responses in humans remain largely undefined. In contrast, T cells have been shown to be essential for viral clearance of mouse norovirus (MNV) infection. In this paper, we demonstrate that CD4(+) T cells secrete gamma interferon (IFN-gamma) in response to stimulation with MNV virus-like particles (VLPs) after MNV infection, supporting earlier reports for norovirus-infected mice and humans. Utilizing this model, we immunized mice with alphavirus vectors (Venezuelan equine encephalitis [VEE] virus replicon particles [VRPs]) expressing Norwalk virus (NV) or Farmington Hills virus (FH) virus-like particles to evaluate T cell epitopes shared between human norovirus strains. Stimulation of splenocytes from norovirus VRP-immunized mice with overlapping peptides from complete libraries of the NV or FH capsid proteins revealed specific amino acid sequences containing T cell epitopes that were conserved within genoclusters and genogroups. Immunization with heterologous norovirus VRPs resulted in specific cross-reactive IFN-gamma secretion profiles following stimulation with NV and FH peptides in the mouse. Identification of unique strain-specific and cross-reactive epitopes may provide insight into homologous and heterologous T cell-mediated norovirus immunity and provide a platform for the study of norovirus-induced cellular immunity in humans. |
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Authors:
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Anna D LoBue; Lisa C Lindesmith; Ralph S Baric |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-06-23 |
Journal Detail:
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Title: Journal of virology Volume: 84 ISSN: 1098-5514 ISO Abbreviation: J. Virol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-06 Completed Date: 2010-09-16 Revised Date: 2011-07-25 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: United States |
Other Details:
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Languages: eng Pagination: 8530-8 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, School of Medicine University of North Carolina at Chapel Hill, North Carolina 27599-7435, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals CD4-Positive T-Lymphocytes / immunology, virology Caliciviridae Infections / immunology*, virology Capsid Proteins / chemistry, genetics, immunology* Cells, Cultured Cross Reactions Epitopes, T-Lymphocyte / chemistry, genetics, immunology* Evolution, Molecular Humans Immunity, Cellular Interferon-gamma / immunology Mice Mice, Inbred BALB C Molecular Sequence Data Norovirus / chemistry, genetics, immunology* Sequence Alignment Species Specificity Spleen / cytology, immunology |
| Grant Support | |
ID/Acronym/Agency:
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AI 056351-06/AI/NIAID NIH HHS; R01 AI056351-08/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Capsid Proteins; 0/Epitopes, T-Lymphocyte; 82115-62-6/Interferon-gamma |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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