| Identification and characterization of a luciferase isotype in the Japanese firefly, Luciola cruciata, involving in the dim glow of firefly eggs. | |
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MedLine Citation:
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PMID: 21090686 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We isolated the cDNA of a luciferase isotype (LcLuc2) from the Japanese firefly, Luciola cruciata (Lampyridae, Coleoptera). The gene product of LcLuc2 (LcLuc2) showed 59% amino acid identity with firefly luciferase LcLuc1, which was previously identified in L. cruciata. The recombinant LcLuc2 showed both luminescence activity and fatty acyl-CoA synthetic activity comparable to those of LcLuc1. The spectral maxima of the luminescence by LcLuc1 and LcLuc2 were 554 and 543 nm, respectively. Reverse transcription-PCR analysis showed that the transcripts of LcLuc1 were abundant in the lanterns of larva, adult male, and adult female, whereas both LcLuc1 and LcLuc2 were expressed in eggs. The luminescence spectra of the lantern extracts from larva, adult male, and adult female were in good agreement with that of recombinant LcLuc1. On the other hand, the emission maximum of the extract from eggs was between those of LcLuc1 and LcLuc2. These results suggest that L. cruciata possesses two luciferases: LcLuc1 is responsible for the major luminescence in larva and adult, whereas LcLuc1 and LcLuc2 are responsible for the dim glow in firefly eggs. |
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Authors:
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Yuichi Oba; Nanae Mori; Mayumi Yoshida; Satoshi Inouye |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-12-03 |
Journal Detail:
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Title: Biochemistry Volume: 49 ISSN: 1520-4995 ISO Abbreviation: Biochemistry Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-21 Completed Date: 2011-01-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: United States |
Other Details:
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Languages: eng Pagination: 10788-95 Citation Subset: IM |
Affiliation:
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Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan. oba@agr.nagoya-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acyl Coenzyme A
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metabolism Amino Acid Sequence Animals DNA, Complementary / genetics, isolation & purification* Female Fireflies / genetics*, metabolism* Gene Expression Introns Luciferases / genetics*, metabolism* Luminescence Male Molecular Sequence Data Phylogeny Protein Isoforms / genetics, metabolism Sequence Alignment |
| Chemical | |
Reg. No./Substance:
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0/Acyl Coenzyme A; 0/DNA, Complementary; 0/Protein Isoforms; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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