Document Detail

Identification and Characterization of In-planta Expressed Secreted Effectors from Magnaporthe oryzae that Induce Cell Death in Rice.
MedLine Citation:
PMID:  23035914     Owner:  NLM     Status:  Publisher    
Interactions between rice and Magnaporthe oryzae involve the recognition of cellular components and the exchange of complex molecular signals from both partners. How these interactions occur in rice cells is still elusive. We employed robust-long serial analysis of gene expression (RL-SAGE), massively parallel signature sequencing (MPSS), and sequencing by synthesis (SBS), to examine transcriptome profiles of infected rice leaves. A total of 6,413 in-planta expressed fungal genes including 851 genes encoding predicted effector proteins were identified. We used a protoplast transient expression system to assess 42 of the predicted effector proteins for the ability to induce plant cell death. Ectopic expression assays identified five novel effectors that induced host cell death only when they contained the signal peptide for secretion to the extracellular space. The five apoplastic effectors induced cell death in a non-host-selective-manner. Although the five effectors are highly diverse in their sequences, the physiological basis of cell death induced by each was similar. This study demonstrates that our integrative genomic approach is effective for the identification of in-planta expressed cell death-inducing effectors from M. oryzae that may play an important role facilitating colonization and fungal growth during infection.
Songbiao Chen; Pattavipha Songkumarn; R Venu; Malali Gowda; Maria Bellizzi; Jinnan Hu; Wende Liu; D Ebbole; Tom Mitchell; Guo-Liang Wang
Related Documents :
4026804 - Decreased ovalbumin-gene response to oestrogen in the prenatally diethylstilboestrol-ex...
8179584 - The ontogeny of dopachrome isomerase isozyme patterns in the tobacco hornworm manduca s...
11073074 - Production of transgenic rabbits using centrifuged pronuclear zygotes.
24577484 - Improved site-specific recombinase-based method to produce selectable marker- and vecto...
22992304 - Cis-regulatory signatures of orthologous stress-associated bzip transcription factors f...
17573724 - Putative association of a tlr9 promoter polymorphism with atopic eczema.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  Molecular plant-microbe interactions : MPMI     Volume:  -     ISSN:  0894-0282     ISO Abbreviation:  Mol. Plant Microbe Interact.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107902     Medline TA:  Mol Plant Microbe Interact     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Institute of Plant Protection, CAAS, Beijing, China;
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Aphid protein effectors promote aphid colonization in a plant species-specific manner.
Next Document:  Toward understanding the role of aphid effectors in plant infestation.