| Identification, characterization, and expression analyses of class II and IV chitinase genes from Douglas-fir seedlings infected by Phellinus sulphurascens. | |
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MedLine Citation:
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PMID: 20205539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Laminated root rot (LRR) disease, caused by the fungus Phellinus sulphurascens, is a major threat to coastal Douglas-fir (DF) (Pseudotsuga menziesii) forests in western North America. Understanding host-pathogen interactions of this pathosystem is essential to manage this important conifer root disease. Our research objectives were to identify DF pathogenesis-related (PR) genes and analyze their expression patterns over the course of infection. We constructed a cDNA library of Phellinus sulphurascens-infected DF seedling roots and sequenced a total of 3,600 random cDNA clones from this library. One of the largest groups of identified genes (203 cDNA clones) matched with chitinase genes reported in other plant species. We identified at least three class II and six class IV chitinase genes from DF seedlings. Quantitative reverse-transcriptase polymerase chain reaction analyses showed significant differential expression patterns locally in root tissues and systemically in needle tissues after fungal invasion. Nonetheless, there was a common trend in gene expression patterns for most of the chitinase genes: an upregulation within 12 h of pathogen inoculation followed by down-regulation within 2 to 3 days postinoculation (dpi), and then further upregulation within 5 to 7 dpi. Western immunoblot data showed differential accumulation of class IV chitinases in Phellinus sulphurascens-infected DF seedlings. Further detailed functional analyses will help us to understand the specific role of DF chitinases in defense against Phellinus sulphurascens infection. |
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Authors:
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M A Islam; R N Sturrock; H L Williams; A K M Ekramoddoullah |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Phytopathology Volume: 100 ISSN: 0031-949X ISO Abbreviation: Phytopathology Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-08 Completed Date: 2010-05-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9427222 Medline TA: Phytopathology Country: United States |
Other Details:
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Languages: eng Pagination: 356-66 Citation Subset: IM |
Affiliation:
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Canadian Forest Service, Pacific Forestry Centre, Victoria, Canada. aislam@nrcan.gc.ca |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chitinase
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genetics,
metabolism Fungal Proteins / genetics, metabolism Fungi / isolation & purification* Gene Expression Regulation, Fungal Phylogeny Plant Diseases / microbiology* Plant Leaves / microbiology Plant Roots / microbiology Pseudotsuga / microbiology* |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; EC 3.2.1.14/Chitinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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