Document Detail


Identification and characterization of a bile salt hydrolase from Lactobacillus salivarius for development of novel alternatives to antibiotic growth promoters.
MedLine Citation:
PMID:  23064348     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antibiotic growth promoters (AGPs) have been used as feed additives to improve average body weight gain and feed efficiency in food animals for more than 5 decades. However, there is a worldwide trend to limit AGP use to protect food safety and public health, which raises an urgent need to discover effective alternatives to AGPs. The growth-promoting effect of AGPs has been shown to be highly correlated with the decreased activity of intestinal bile salt hydrolase (BSH), an enzyme that is produced by various gut microflora and involved in host lipid metabolism. Thus, BSH inhibitors are likely promising feed additives to AGPs to improve animal growth performance. In this study, the genome of Lactobacillus salivarius NRRL B-30514, a BSH-producing strain isolated from chicken, was sequenced by a 454 GS FLX sequencer. A BSH gene identified by genome analysis was cloned and expressed in an Escherichia coli expression system for enzymatic analyses. The BSH displayed efficient hydrolysis activity for both glycoconjugated and tauroconjugated bile salts, with slightly higher catalytic efficiencies (k(cat)/K(m)) on glycoconjugated bile salts. The optimal pH and temperature for the BSH activity were 5.5 and 41°C, respectively. Examination of a panel of dietary compounds using the purified BSH identified some potent BSH inhibitors, in which copper and zinc have been recently demonstrated to promote feed digestion and body weight gain in different food animals. In sum, this study identified and characterized a BSH with broad substrate specificity from a chicken L. salivarius strain and established a solid platform for us to discover novel BSH inhibitors, the promising feed additives to replace AGPs for enhancing the productivity and sustainability of food animals.
Authors:
Zhong Wang; Ximin Zeng; Yiming Mo; Katie Smith; Yuming Guo; Jun Lin
Related Documents :
22071608 - Development of a multiplex dna-based traceability tool for crop plant materials.
22251738 - Sampling efficacy for the red imported fire ant solenopsis invicta (hymenoptera: formic...
23756158 - Effect of neuropeptide y on food intake in bullfrog larvae.
21689778 - Phylogenetic constraints on digesta separation: variation in fluid throughput in the di...
24475538 - Brain food: bringing psychological insights to bear on modern nutrition labeling efforts.
21180988 - Focused principal component analysis: a graphical method for exploring dietary patterns.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-12
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-19     Completed Date:  2013-04-22     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8795-802     Citation Subset:  IM    
Affiliation:
Department of Animal Science, University of Tennessee, Knoxville, Tennessee, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/JX120368;  JX120369
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amidohydrolases / genetics,  isolation & purification*,  metabolism*
Animals
Anti-Bacterial Agents / metabolism*
Bile Acids and Salts / metabolism
Chickens / microbiology
Cloning, Molecular
DNA, Bacterial / chemistry,  genetics
Enzyme Activators / metabolism
Escherichia coli / genetics
Food Additives / metabolism*
Gene Expression
Hydrogen-Ion Concentration
Intercellular Signaling Peptides and Proteins / metabolism*
Kinetics
Lactobacillus / enzymology*,  genetics,  isolation & purification
Metals / metabolism
Molecular Sequence Data
Promoter Regions, Genetic
Recombinant Proteins / genetics,  isolation & purification,  metabolism
Sequence Analysis, DNA
Temperature
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bile Acids and Salts; 0/DNA, Bacterial; 0/Enzyme Activators; 0/Food Additives; 0/Intercellular Signaling Peptides and Proteins; 0/Metals; 0/Recombinant Proteins; EC 3.5.-/Amidohydrolases; EC 3.5.1.24/choloylglycine hydrolase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Protective role of spore structural components in determining Bacillus subtilis spore resistance to ...
Next Document:  Domibacillus robiginosus gen. nov., sp. nov., isolated from a pharmaceutical clean room.