| Identification of Candida albicans and Candida glabrata within 1.5 hours directly from positive blood culture bottles with a shortened peptide nucleic acid fluorescence in situ hybridization protocol. | |
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MedLine Citation:
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PMID: 18987173 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Candida albicans and Candida glabrata can be identified in blood culture bottles within 2.5 h using peptide nucleic acid fluorescence in situ hybridization. A 1.25-h protocol was compared to the standard with 40 positive (clinical and spiked) blood culture bottles tested in batches of 5. All C. albicans (15) and C. glabrata (16) isolates, alone or mixed, were identified correctly using both protocols, whereas 18 isolates (five other species) were negative by both protocols. This shortened method will significantly reduce the time to identification. |
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Authors:
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Michael Gherna; William G Merz |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article Date: 2008-11-05 |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 47 ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-01-05 Completed Date: 2009-02-02 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 247-8 Citation Subset: IM |
Affiliation:
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Department of Pathology, the Johns Hopkins Medical Institutions, Baltimore, Maryland, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blood
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microbiology* Candida albicans / isolation & purification* Candida glabrata / isolation & purification* Humans In Situ Hybridization, Fluorescence / methods* Mycology / methods* Peptide Nucleic Acids / diagnostic use* Sensitivity and Specificity Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Peptide Nucleic Acids |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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