Document Detail


Identification of Campylobacter jejuni genes contributing to acid adaptation by transcriptional profiling and genome-wide mutagenesis.
MedLine Citation:
PMID:  18192408     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to cause disease, the food- and waterborne pathogen Campylobacter jejuni must face the extreme acidity of the host stomach as well as cope with pH fluctuations in the intestine. In the present study, C. jejuni NCTC 11168 was grown under mildly acidic conditions mimicking those encountered in the intestine. The resulting transcriptional profiles revealed how this bacterium fine-tunes gene expression in response to acid stress. This adaptation involves the differential expression of respiratory pathways, the induction of genes for phosphate transport, and the repression of energy generation and intermediary metabolism genes. We also generated and screened a transposon-based mutant library to identify genes required for wild-type levels of growth under mildly acidic conditions. This screen highlighted the important role played by cell surface components (flagella, the outer membrane, capsular polysaccharides, and lipooligosaccharides) in the acid stress response of C. jejuni. Our data also revealed that a limited correlation exists between genes required for growth under acidic conditions and genes differentially expressed in response to acid. To gain a comprehensive picture of the acid stress response of C. jejuni, we merged transcriptional profiles obtained from acid-adapted cells and cells subjected to acid shock. Genes encoding the transcriptional regulator PerR and putative oxidoreductase subunits Cj0414 and Cj0415 were among the few up-regulated under both acid stress conditions. As a Cj0415 mutant was acid sensitive, it is likely that these genes are crucial to the acid stress response of C. jejuni and consequently are important for host colonization.
Authors:
Anne N Reid; Reenu Pandey; Kiran Palyada; Lisa Whitworth; Evgueni Doukhanine; Alain Stintzi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-01-11
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  74     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-26     Completed Date:  2008-03-31     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1598-612     Citation Subset:  IM    
Affiliation:
Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 451 Smyth Road, Ottawa, Ontario K1H 8M5, Canada.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological / genetics*
Alkanesulfonates
Bacterial Proteins / genetics
Campylobacter jejuni / genetics*,  metabolism
DNA Primers / genetics
Gene Expression Profiling
Gene Expression Regulation, Bacterial*
Gene Library
Hydrogen-Ion Concentration
Microarray Analysis
Mutagenesis
Phenotype*
Repressor Proteins / genetics
Transcription Factors / genetics
Grant Support
ID/Acronym/Agency:
R01-AI055612/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Alkanesulfonates; 0/Bacterial Proteins; 0/DNA Primers; 0/Repressor Proteins; 0/Transcription Factors; 0/peroxide repressor proteins; 594-45-6/ethane sulfonate
Comments/Corrections

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