Document Detail

Identification of CSF-1 as a brain macrophage migratory activity produced by astrocytes.
MedLine Citation:
PMID:  9728764     Owner:  NLM     Status:  MEDLINE    
Intraparenchymal migration of macrophages occurs in the CNS during development or as a consequence of tissue injuries. In the present study, we have shown, by using an in vitro chemotaxis assay, that cultured rat astrocytes obtained from the developing cerebral cortex and striatum produce soluble factors, which attract purified brain macrophages. The effect of astrocyte-derived factors on macrophages was strongly reduced in the presence of antibodies neutralizing colony-stimulating factor 1 (CSF-1, also called M-CSF), and recombinant CSF-1 was found to act as a chemotactic agent on brain macrophages. Synthesis of CSF-1 by cultured astrocytes was confirmed by northern detection of CSF-1 transcripts. In contrast, the CSF-1 gene was not expressed by cultured neurons from the cerebral cortex and striatum or by the brain macrophage population responsive to CSF-1 gradient. ELISA detection of CSF-1 in tissue extracts revealed the occurrence of this cytokine in the rat cerebral cortex during postnatal development and in adults. Altogether, our results demonstrate that astrocytes, through CSF-1 secretion, can trigger the polarized migration of brain macrophages and suggest a new mechanism which could regulate the locomotion of these cells in the cerebral cortex during ontogenesis or following lesions.
C F Calvo; A Dobbertin; M Gelman; J Glowinski; M Mallat
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glia     Volume:  24     ISSN:  0894-1491     ISO Abbreviation:  Glia     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-11-30     Completed Date:  1998-11-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8806785     Medline TA:  Glia     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  180-6     Citation Subset:  IM    
INSERM U114, Collège de France, Paris.
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MeSH Terms
Astrocytes / metabolism*
Blotting, Northern
Brain / cytology*
Brain Chemistry / physiology
Cell Division / physiology
Cell Movement / physiology
Cell Survival / physiology
Cells, Cultured
Chemotaxis, Leukocyte / physiology
Cytokines / biosynthesis
Enzyme-Linked Immunosorbent Assay
Macrophage Colony-Stimulating Factor / biosynthesis*,  physiology*
Macrophages / metabolism*
Neurons / metabolism
Reg. No./Substance:
0/Cytokines; 81627-83-0/Macrophage Colony-Stimulating Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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