| Identification of CD36 as a new surface marker of marginal zone B cells by transcriptomic analysis. | |
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MedLine Citation:
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PMID: 16616782 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Follicular (FO) B cells and marginal zone (MZ) B cells belong to the mature B cell population in spleen of mice. To identify new surface markers of these mature B cell subsets, we compared gene expression profiles of FO and MZ B cells by DNA microarray using FACS-sorted mouse FO and MZ B cells. From 14,000 mouse genes, 27 membrane proteins were expressed mainly in MZ B cells while another 22 membrane proteins expressed largely in FO B cells. Using FACS analysis, we identified that CD36, CD68, and CD49e were expressed on MZ B cells but not on FO B cells. In addition, using semi-quantitative PCR, we found that the mRNA of CD131 were much more abundant in MZ B cells. These results revealed new phenotypic properties of MZ and FO B cells, and would facilitate further studies in the differentiation and functions of these mature B cells. |
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Authors:
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Ping Zhang; Wei Li; Yaochun Wang; Lihong Hou; Ying Xing; Hongyan Qin; Jishu Wang; Yingmin Liang; Hua Han |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-04-17 |
Journal Detail:
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Title: Molecular immunology Volume: 44 ISSN: 0161-5890 ISO Abbreviation: Mol. Immunol. Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2006-08-29 Completed Date: 2007-02-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7905289 Medline TA: Mol Immunol Country: England |
Other Details:
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Languages: eng Pagination: 332-7 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Cancer Biology, Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, and Department of Dermatology, Xijng Hospital, Xi'an 710032, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD36 / biosynthesis, genetics, immunology* Antigens, Surface / immunology B-Lymphocyte Subsets / immunology*, metabolism Biological Markers Female Flow Cytometry Gene Expression Profiling Mice Mice, Inbred C57BL Spleen / cytology, immunology Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD36; 0/Antigens, Surface; 0/Biological Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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