Document Detail


Identification of CD36 as a new surface marker of marginal zone B cells by transcriptomic analysis.
MedLine Citation:
PMID:  16616782     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Follicular (FO) B cells and marginal zone (MZ) B cells belong to the mature B cell population in spleen of mice. To identify new surface markers of these mature B cell subsets, we compared gene expression profiles of FO and MZ B cells by DNA microarray using FACS-sorted mouse FO and MZ B cells. From 14,000 mouse genes, 27 membrane proteins were expressed mainly in MZ B cells while another 22 membrane proteins expressed largely in FO B cells. Using FACS analysis, we identified that CD36, CD68, and CD49e were expressed on MZ B cells but not on FO B cells. In addition, using semi-quantitative PCR, we found that the mRNA of CD131 were much more abundant in MZ B cells. These results revealed new phenotypic properties of MZ and FO B cells, and would facilitate further studies in the differentiation and functions of these mature B cells.
Authors:
Ping Zhang; Wei Li; Yaochun Wang; Lihong Hou; Ying Xing; Hongyan Qin; Jishu Wang; Yingmin Liang; Hua Han
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-04-17
Journal Detail:
Title:  Molecular immunology     Volume:  44     ISSN:  0161-5890     ISO Abbreviation:  Mol. Immunol.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-08-29     Completed Date:  2007-02-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905289     Medline TA:  Mol Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  332-7     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Cancer Biology, Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, and Department of Dermatology, Xijng Hospital, Xi'an 710032, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD36 / biosynthesis,  genetics,  immunology*
Antigens, Surface / immunology
B-Lymphocyte Subsets / immunology*,  metabolism
Biological Markers
Female
Flow Cytometry
Gene Expression Profiling
Mice
Mice, Inbred C57BL
Spleen / cytology,  immunology
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Antigens, CD36; 0/Antigens, Surface; 0/Biological Markers

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