Document Detail


Identification of a CD2- CD3+ T cell receptor-gamma+ peripheral blood lymphocyte subpopulation.
MedLine Citation:
PMID:  2895149     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have identified, in a healthy individual, a sub-population of human peripheral lymphocytes which surface express a CD3-TCR-gamma complex recognized by anti-Ti gamma A mAb, while being unreactive with a phycoerythrin-conjugated anti-CD2 antibody with T11/1 specificity. Further immunofluorescence analyses performed on uncultured cells indicated that such a putative CD2-CD3+ phenotype was restricted to a fraction of those T lymphocytes which carry a surface receptor of the "second family" (gamma/delta). The actual lack of CD2 expression was confirmed by a subsequent series of cloning experiments which showed that none of the three well characterized CD2 epitopic clusters, namely T11/1, T11/2, and T11/3, were detectable on the surface of the relevant cells. The cultured CD2-, CD3+/TCR gamma + lymphocytes were found to display, as well as their CD2+ counterparts, both non-MHC-restricted cytotoxic function and proliferative responses induced via the gamma receptor complex. In contrast, the proliferative capacity of the CD2-, CD3+/TCR-gamma + cells observed in a culture system designed for in vitro expansion of lymphocytes with undefined specificity was extremely limited. This may relate to an impaired interaction of the CD2- cloned lymphocytes with lymphocyte function-associated (LFA)3+ irradiated cells present in the feeder layer. Further characterization of such minor CD2- T lymphocytes subsets may help to better understand the biologic relevance of the CD2/LFA3 pathway of cell-cell interaction.
Authors:
F Faure; S Jitsukawa; S Meuer; C Bohuon; F Triebel; T Hercend
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  140     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1988 Apr 
Date Detail:
Created Date:  1988-04-26     Completed Date:  1988-04-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2128-32     Citation Subset:  AIM; IM    
Affiliation:
Département de Biologie Clinique, Institut Gustave-Roussy, Villejuif, France.
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Antibodies, Monoclonal / immunology
Antigen-Antibody Reactions
Antigens, CD2
Antigens, Differentiation, T-Lymphocyte / analysis*,  immunology
Carrier Proteins / analysis,  immunology
Clone Cells / classification,  immunology,  metabolism
Cytotoxicity, Immunologic
Fluorescent Antibody Technique
Humans
Mice
Phenotype
Receptors, Antigen, T-Cell / analysis*,  immunology
Receptors, Immunologic / analysis,  immunology
T-Lymphocytes / classification*,  immunology,  metabolism
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD2; 0/Antigens, Differentiation, T-Lymphocyte; 0/Carrier Proteins; 0/Receptors, Antigen, T-Cell; 0/Receptors, Immunologic

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