Document Detail


Identification of CD16-2, a novel mouse receptor homologous to CD16/Fc gamma RIII.
MedLine Citation:
PMID:  12389094     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It is believed that mouse Fc gamma RIII arose by an evolutionarily recent recombination, which brought together the extracellular domains from Fc gamma RII with the transmembrane/cytoplasmic region from the ancestor Fc gamma RIII. Here, we report identification of a mouse gene encoding a transmembrane receptor that may be regarded as the true ortholog of nonrodent CD16/Fc gamma RIII. Designated CD16-2, the novel protein is highly similar to human Fc gamma RIIIA in the signal peptide (60% identical residues), and in the extracellular domains (65%). Although the similarity between the two proteins is less conspicuous in the transmembrane/cytoplasmic region (54%), it is higher than between human Fc gamma RIIIA and mouse Fc gamma RIII (44%). However, the conserved transmembrane motif LFAVDTGL shared by rodent and human Fc gamma RIII and Fc epsilon RI has two replacements in CD16-2. The CD16-2 gene is tightly linked to the Fc gamma RIII and Fc gamma RII genes and consists of five exons. Northern blot analysis revealed that CD16-2 is expressed in peripheral blood leukocytes, as well as in spleen, thymus, colon and intestine. RT-PCR showed prominent expression in macrophage cell line J774. Based on sequence comparisons, it is suggested that the modern repertoire of the mammalian low affinity Fc receptors has resulted from repetitive duplications and/or recombinations of three ancestral genes.
Authors:
Ludmila V Mechetina; Alexander M Najakshin; Boris Y Alabyev; Nikolai A Chikaev; Alexander V Taranin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-08-02
Journal Detail:
Title:  Immunogenetics     Volume:  54     ISSN:  0093-7711     ISO Abbreviation:  Immunogenetics     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-10-21     Completed Date:  2003-01-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0420404     Medline TA:  Immunogenetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  463-8     Citation Subset:  IM    
Affiliation:
Laboratory of Immunogenetics, Institute of Cytology and Genetics, Lavrentjev st. 10, Novosibirsk 630090, Russia.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Humans
Mice
Molecular Sequence Data
Phylogeny
Receptors, IgG / chemistry,  genetics*
Sequence Alignment
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/Receptors, IgG

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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