Document Detail

Identification of a CA repeat at the TCRA locus using yeast artificial chromosomes: a general method for generating highly polymorphic markers at chosen loci.
MedLine Citation:
PMID:  1386339     Owner:  NLM     Status:  MEDLINE    
The creation of a comprehensive genetic map in human has been limited by the lack of highly polymorphic markers spaced evenly throughout the human genome. We have utilized yeast artificial chromosomes (YAC) containing large human DNA inserts to help identify highly polymorphic (CA)n repeats at a chosen locus. The DNA of a YAC containing the locus was subcloned in M13 vectors, and the recombinants were screened at high stringency to detect preferentially long (CA)n repeats (n greater than 20). These repeats, which are the most likely to be highly polymorphic, were then studied to confirm both the level of polymorphism and their precise genetic location. This strategy has permitted the identification of a new, highly polymorphic CA repeat (77% heterozygosity) at the T cell receptor alpha chain (TCRA) locus on chromosome 14q. It provides a powerful marker for assessing the role of this locus in the susceptibility to autoimmune and infectious diseases. This approach should permit the development of highly polymorphic markers at any targeted locus and rapidly improve the current human genetic map.
F Cornélis; L Hashimoto; J Loveridge; A MacCarthy; V Buckle; C Julier; J Bell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genomics     Volume:  13     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-08-28     Completed Date:  1992-08-28     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  820-5     Citation Subset:  IM    
Molecular Immunology Group, Institute of Molecular Medicine, Oxford, United Kingdom.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M84017;  M84534;  M84535;  M84536;  M84537;  M84538;  M84539;  M84543;  M84544;  S41488;  X60596
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MeSH Terms
Base Sequence
Chromosomes, Fungal
DNA / genetics
DNA Probes
DNA, Satellite / genetics
Gene Library
Genetic Markers*
Genome, Human
Molecular Sequence Data
Polymorphism, Genetic
Receptors, Antigen, T-Cell, alpha-beta / genetics*
Repetitive Sequences, Nucleic Acid*
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/DNA Probes; 0/DNA, Satellite; 0/Genetic Markers; 0/Receptors, Antigen, T-Cell, alpha-beta; 9007-49-2/DNA

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