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Identification of the Biochemical Degradation Pathway of Triazophos and its Intermediate in Diaphorobacter sp. TPD-1.
MedLine Citation:
PMID:  21212954     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Triazophos is one of the most widely used organophosphorus insecticides usually detectable in the environment. A bacterial strain, Diaphorobacter sp. TPD-1, capable of using triazophos and its intermediate, 1-phenyl-3-hydroxy-1,2,4-triazole (PHT), as its sole carbon sources for growth was isolated from a triazophos-contaminated soil in China. This strain could completely degrade 50 mg l(-1) triazophos and PHT to non-detectable level in 24 and 56 h, respectively. During PHT degradation, three metabolites were detected and identified based on tandem mass spectrometry (MS/MS) analysis. Using this information, a biochemical degradation pathway of triazophos by Diaphorobacter sp. TPD-1 was proposed. The first step involved in the degradation of triazophos is the hydrolysis of the P-O ester bond of triazophos to form PHT and o,o-diethyl phosphorothioic acid, then the triazol ring of PHT is subsequently cleaved to form (E)-1-formyl-2-phenyldiazene. Subsequently, (E)-1-formyl-2-phenyldiazene is transformed to 2-phenylhydrazinecarboxylic acid by adding one molecular of H(2)O. Finally, the carboxyl group of 2-phenylhydrazinecarboxylic acid is decarboxylated to form phenylhydrazine.
Authors:
Chengli Yang; Rong Li; Yao Song; Kai Chen; Shunpeng Li; Jiandong Jiang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-1-7
Journal Detail:
Title:  Current microbiology     Volume:  -     ISSN:  1432-0991     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-1-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Microbiology, Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
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