| Identification of BSPRY as a novel auxiliary protein inhibiting TRPV5 activity. | |
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MedLine Citation:
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PMID: 16380433 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transient receptor potential vallinoid 5 (TRPV5) and TRPV6 are the most Ca2+-selective members of the TRP superfamily and are essential for active Ca2+ (re)absorption in epithelia. However, little is known about intracellular proteins that regulate the activity of these channels. This study identified BSPRY (B-box and SPRY-domain containing protein) as a novel factor involved in the control of TRPV5. The interaction between BSPRY and TRPV5 by GST pull-down and co-immunoprecipitation assays was demonstrated. BSPRY showed co-localization with TRPV5 in mouse kidney. Expression of BSPRY resulted in a significant reduction of the Ca2+ influx in Madin-Darby Canine Kidney cells that stably express TRPV5 without affecting channel cell-surface abundance. Finally, BSPRY expression in kidney was increased in 25-hydroxyvitamin D3-1alpha-hydroxylase knockout mice, suggesting an inverse regulation by vitamin D3. Together, these results demonstrate the physiologic role of the novel protein BSPRY in the regulation of epithelial Ca2+ transport via negative modulation of TRPV5 activity. |
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Authors:
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Stan F J van de Graaf; Annemiete W C M van der Kemp; Dennis van den Berg; Mijke van Oorschot; Joost G J Hoenderop; René J M Bindels |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of the American Society of Nephrology : JASN Volume: 17 ISSN: 1046-6673 ISO Abbreviation: J. Am. Soc. Nephrol. Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2005-12-28 Completed Date: 2006-06-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9013836 Medline TA: J Am Soc Nephrol Country: United States |
Other Details:
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Languages: eng Pagination: 26-30 Citation Subset: IM |
Affiliation:
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Cell Physiology, Radboud University Nijmegen Medical Centre, PO Box 9101, NL-6500 HB Nijmegen, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Calcitriol / physiology Calcium / metabolism Calcium Channels / analysis, physiology Cell Line Molecular Sequence Data Proteins / analysis, chemistry, physiology* TRPV Cation Channels / analysis, antagonists & inhibitors*, physiology Xenopus |
| Chemical | |
Reg. No./Substance:
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0/BSPRY protein, human; 0/Calcium Channels; 0/Proteins; 0/TRPV Cation Channels; 0/Trpv5 protein, mouse; 32222-06-3/Calcitriol; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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