Document Detail


Identification of a 70 kD protein with sequence homology to squid neurofilament protein in glial cells of the leech CNS.
MedLine Citation:
PMID:  8113784     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A monoclonal antibody G39, generated against a protein extract of leech central nervous system, labels specific cell types in adult, embryonic, and regenerating preparations. The antibody stained glial cells, microglial cells, and connective tissue cells, but not neurons or muscle on cryosections. The staining pattern resembled that of an intracellular network. Affinity purification of the antigen revealed a 70 kD protein. Peptide sequencing showed significant homology of a stretch of 15 amino acids to squid neural filament protein. The same mAb G39 delineated glial cells as they formed during development of the CNS and showed that the giant neuropil glial cells appear before those in the packets. The antigen recognized by mAb G39 represents a nonneuronal intermediate filament of the leech Hirudo medicinalis found in various cell-types such as glia, microglia, and some cells of the connective tissue.
Authors:
T E Lüthi; D L Brodbeck; P Jenö
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurobiology     Volume:  25     ISSN:  0022-3034     ISO Abbreviation:  J. Neurobiol.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-03-31     Completed Date:  1994-03-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0213640     Medline TA:  J Neurobiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  70-82     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Universität Basel, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Monoclonal / immunology
Blotting, Western
Central Nervous System / cytology,  metabolism
Concanavalin A / chemistry
Decapodiformes / metabolism*
Electrophoresis, Polyacrylamide Gel
Hydrogen-Ion Concentration
Immunohistochemistry
Leeches / metabolism*
Mice
Mice, Inbred BALB C / immunology
Molecular Sequence Data
Nerve Crush
Neurofilament Proteins / analysis*
Neuroglia / chemistry*
Sequence Homology, Amino Acid*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Neurofilament Proteins; 11028-71-0/Concanavalin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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