| Identification of the 22-phosphate esters of ecdysone, 2-deoxyecdysone, 20-hydroxyecdysone and 2-deoxy-20-hydroxyecdysone from newly laid eggs of the desert locust, Schistocerca gregaria. | |
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MedLine Citation:
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PMID: 6684423 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The four major ecdysteroid (insect moulting hormone) conjugates present in the newly laid eggs of the desert locust, Schistocera gregaria, have been purified by reversed-phase and anion-exchange high-performance liquid chromatography. The steroid moieties were identified as ecdysone, 2-deoxyecdysone, 20-hydroxyecdysone and 2-deoxy-20-hydroxyecdysone. Phosphate analysis of acid-hydrolysed samples showed a steroid:phosphate ratio of approx. 1:1 for all four compounds. The intact conjugates were identified as ecdysone 22-phosphate, 2-deoxyecdysone 22-phosphate, 20-hydroxyecdysone 22-phosphate and 2-deoxy-20-hydroxyecdysone 22-phosphate by fast atom bombardment mass spectrometry and 1H, 13C and 31P n.m.r. The significance of ecdysteroid phosphates as a source of free hormone during embryogenesis is discussed. |
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Authors:
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R E Isaac; M E Rose; H H Rees; T W Goodwin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 213 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1983 Aug |
Date Detail:
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Created Date: 1983-10-08 Completed Date: 1983-10-08 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 533-41 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, High Pressure Liquid Ecdysone / analogs & derivatives*, analysis Female Grasshoppers / analysis* Magnetic Resonance Spectroscopy Mass Spectrometry Ovum / analysis* |
| Chemical | |
Reg. No./Substance:
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3604-87-3/Ecdysone; 82183-62-8/ecdysone-22-phosphate; 82183-63-9/2-deoxyecdysone-22-phosphate; 86577-96-0/2-deoxy-20-hydroxyecdysone-22-phosphate; 86577-97-1/20-hydroxyecdysone-22-phosphate |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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