Document Detail


Identification of the 22-phosphate esters of ecdysone, 2-deoxyecdysone, 20-hydroxyecdysone and 2-deoxy-20-hydroxyecdysone from newly laid eggs of the desert locust, Schistocerca gregaria.
MedLine Citation:
PMID:  6684423     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The four major ecdysteroid (insect moulting hormone) conjugates present in the newly laid eggs of the desert locust, Schistocera gregaria, have been purified by reversed-phase and anion-exchange high-performance liquid chromatography. The steroid moieties were identified as ecdysone, 2-deoxyecdysone, 20-hydroxyecdysone and 2-deoxy-20-hydroxyecdysone. Phosphate analysis of acid-hydrolysed samples showed a steroid:phosphate ratio of approx. 1:1 for all four compounds. The intact conjugates were identified as ecdysone 22-phosphate, 2-deoxyecdysone 22-phosphate, 20-hydroxyecdysone 22-phosphate and 2-deoxy-20-hydroxyecdysone 22-phosphate by fast atom bombardment mass spectrometry and 1H, 13C and 31P n.m.r. The significance of ecdysteroid phosphates as a source of free hormone during embryogenesis is discussed.
Authors:
R E Isaac; M E Rose; H H Rees; T W Goodwin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  213     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1983 Aug 
Date Detail:
Created Date:  1983-10-08     Completed Date:  1983-10-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  533-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, High Pressure Liquid
Ecdysone / analogs & derivatives*,  analysis
Female
Grasshoppers / analysis*
Magnetic Resonance Spectroscopy
Mass Spectrometry
Ovum / analysis*
Chemical
Reg. No./Substance:
3604-87-3/Ecdysone; 82183-62-8/ecdysone-22-phosphate; 82183-63-9/2-deoxyecdysone-22-phosphate; 86577-96-0/2-deoxy-20-hydroxyecdysone-22-phosphate; 86577-97-1/20-hydroxyecdysone-22-phosphate
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