Document Detail


Identification of a 21 amino-acid peptide conferring 3-hydroxypropionic acid stress-tolerance to Escherichia coli.
MedLine Citation:
PMID:  22161628     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We report the identification of a novel small open reading frame in Escherichia coli. The sORF (called iroK) encodes a 21 amino-acid peptide, which when translated confers a 133% (c.a. 20 g/L) increase in resistance to 3-hydroxypropionic acid. We show that IroK conferred tolerance is additive to previously identified tolerance mechanisms involving relief of inhibited metabolism, yet does not involve altered 3HP transport. This result demonstrates the continued surprises that microbial genomes hold and emphasize the importance of comprehensive discovery methods in future strain and metabolic engineering efforts. Biotechnol. Bioeng. © 2011 Wiley Periodicals, Inc.
Authors:
T E Warnecke; M D Lynch; M L Lipscomb; R T Gill
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Publication Detail:
Type:  LETTER     Date:  2011-12-7
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  -     ISSN:  1097-0290     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Wiley Periodicals, Inc.
Affiliation:
OPX Biotechnologies, Boulder, CO 80301; Department of Chemical and Biological Engineering and Colorado Center for Biorefining and Biofuels, University of Colorado, Boulder, CO 80309.
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