| Idebenone-induced recovery of glycerol-3-phosphate and succinate oxidation inhibited by digitonin. | |
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MedLine Citation:
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PMID: 22480420 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Digitonin solubilizes mitochondrial membrane, breaks the integrity of the respiratory chain and releases two mobile redox-active components: coenzyme Q (CoQ) and cytochrome c (cyt c). In the present study we report the inhibition of glycerol-3-phosphate- and succinate-dependent oxygen consumption rates by digitonin treatment. Our results show that the inhibition of oxygen consumption rates is recovered by the addition of exogenous synthetic analog of CoQ idebenone (hydroxydecyl-ubiquinone; IDB) and cyt c. Glycerol-3-phosphate oxidation rate is recovered to 148 % of control values, whereas succinate-dependent oxidation rate only to 68 %. We find a similar effect on the activities of glycerol-3-phosphate and succinate cytochrome c oxidoreductase. Our results also indicate that succinate-dependent oxidation is less sensitive to digitonin treatment and less activated by IDB in comparison with glycerol-3-phosphate-dependent oxidation. These findings might indicate the different mechanism of the electron transfer from two flavoprotein-dependent dehydrogenases (glycerol-3-phosphate dehydrogenase and succinate dehydrogenase) localized on the outer and inner face of the inner mitochondrial membrane, respectively. |
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Authors:
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H Rauchová; M Vokurková; Z Drahota |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-5 |
Journal Detail:
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Title: Physiological research / Academia Scientiarum Bohemoslovaca Volume: - ISSN: 1802-9973 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9112413 Medline TA: Physiol Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic. rauchova@biomed.cas.cz. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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