Document Detail

Ideal ratios of isoleucine, methionine, methionine plus cystine, threonine, tryptophan, and valine relative to lysine for white leghorn-type laying hens of twenty-eight to thirty-four weeks of age.
MedLine Citation:
PMID:  18339997     Owner:  NLM     Status:  MEDLINE    
Seven separate experiments were conducted with Hy-Line W-36 hens to determine the ideal ratio of Arg, Ile, Met, Met+Cys, Thr, Trp, and Val relative to Lys for maximal egg mass. The experiments were conducted simultaneously and were each designed as a randomized complete block design with 60 experimental units (each consisting of 1 cage with 2 hens) and 5 dietary treatments. The 35 assay diets were made from a common basal diet (2,987 kcal/kg of ME; 12.3% CP; 4.06% Ca, 0.47% nonphytate P), formulated using corn, soybean meal, and meat and bone meal. The true digestible amino acid contents in the basal diet were determined using the precision-fed assay with adult cecectomized roosters. Crystalline L-Arg (free base), L-Ile, L-Lys.HCl, DL-Met, L-Thr, L-Trp, and L-Val (considered 100% true digestible) were added to the basal diet at the expense of cornstarch to make the respective assayed amino acid first limiting and to yield 5 graded inclusions of the assayed amino acid. Hens were fed the assay diets from 26 to 34 wk of age, with the first 2 wk considered a depletion period. Egg production was recorded daily and egg weight was determined weekly on eggs collected over 48 h; egg mass was calculated as egg production x egg weight. The requirement for each amino acid was determined using the broken-line regression method. Consumption of Arg did not affect egg mass, thus a requirement could not be determined. The true digestible amino acid requirements used to calculate the ideal amino acid ratio for maximum egg mass were 426 mg/d of Ile, 538 mg/d of Lys, 253 mg/d of Met, 506 mg/d of Met+Cys, 414 mg/d of Thr, 120 mg/d of Trp, and 501 mg/d of Val. The ideal amino acid ratio for maximum egg mass was Ile 79%, Met 47%, Met+Cys 94%, Thr 77%, Trp 22%, and Val 93% on a true digestible basis relative to Lys. The ideal Met and Met+Cys ratios were verified in an ensuing identical experiment with 52- to 58-wk-old hens.
K Bregendahl; S A Roberts; B Kerr; D Hoehler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Poultry science     Volume:  87     ISSN:  0032-5791     ISO Abbreviation:  Poult. Sci.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-14     Completed Date:  2008-07-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0401150     Medline TA:  Poult Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  744-58     Citation Subset:  IM    
Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
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MeSH Terms
Amino Acids / administration & dosage*,  metabolism
Animal Feed
Animal Nutritional Physiological Phenomena*
Body Weight / physiology
Chickens / metabolism,  physiology*
Eating / physiology
Oviposition / physiology*
Random Allocation
Regression Analysis
Reg. No./Substance:
0/Amino Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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