Document Detail

Ideal culture time for improvement in sperm motility from testicular sperm aspirates of men with azoospermia.
MedLine Citation:
PMID:  17869289     Owner:  NLM     Status:  MEDLINE    
PURPOSE: The motility of testicular derived spermatozoa reflects viability and predicts success during intracytoplasmic sperm injection. Although improvements in sperm motility are seen after incubation for extended periods, no guidelines suggest duration or media use for optimal improvement in motility. MATERIALS AND METHODS: Between July 1999 and February 2005 testicular aspirations were performed on 95 men with azoospermia, including 51 with obstructive azoospermia and 44 with nonobstructive azoospermia. Sperm motility was determined at initial collection and following incubation for 24 or 48 hours in processing media or Ham's F10 + protein. A mixed regression model controlling for testis side, media and baseline motility was created to analyze the change in motility between 24 and 48 hours. RESULTS: Mean motility improved from 3% to 20% at 24 hours and 25% at 48 hours for OA cases and from 0% to 5% at 24 hours and 11% at 48 hours for nonobstructive azoospermia cases. The improvement in motility from 24 to 48 hours was significant for obstructive azoospermia cases (p = 0.001). While media was a nonsignificant factor in regression models, when patients were grouped into categories of motility change there was a significantly better response to F10 compared to processing media (p = 0.03). CONCLUSIONS: Incubation in processing media or Ham's F10 + albumin media improves sperm motility with significant improvement noted between 24 and 48 hours for obstructive azoospermia cases. Ham's F10 + albumin media may provide extra benefit for cases of nonobstructive azoospermia or nerve injury. These results suggest the ideal timing of oocyte retrieval for intracytoplasmic sperm injection correlates with 48-hour sperm incubation for obstructive azoospermia cases, and 24 hours for nonobstructive azoospermia and nerve injury cases.
David S Morris; Rodney L Dunn; Timothy G Schuster; Dana A Ohl; Gary D Smith
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Publication Detail:
Type:  Journal Article     Date:  2007-09-17
Journal Detail:
Title:  The Journal of urology     Volume:  178     ISSN:  0022-5347     ISO Abbreviation:  J. Urol.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-15     Completed Date:  2007-11-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376374     Medline TA:  J Urol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2087-91; discussion 2091     Citation Subset:  AIM; IM    
Department of Urology, University of Michigan, Ann Arbor, Michigan 48109-0617, USA.
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MeSH Terms
Azoospermia / pathology,  physiopathology*
Cells, Cultured / physiology
Culture Media / standards*
Retrospective Studies
Semen Preservation / standards*
Sperm Motility / physiology*
Spermatozoa / physiology*
Time Factors
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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