| Id2a influences neuron and glia formation in the zebrafish retina by modulating retinoblast cell cycle kinetics. | |
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MedLine Citation:
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PMID: 20943708 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inhibitor of differentiation (Id) family helix-loop-helix proteins regulate the proliferation, survival and differentiation of numerous cell types during development; however, their functions during retinal development have not been analyzed. Using loss-of-function and overexpression assays in zebrafish, we demonstrate that Id2a levels modulate retinoblast cell cycle kinetics and thereby influence neuron and glia formation in the retina. Id2a-deficient retinas possess increased numbers of cells occupying S phase, at the expense of mitotic cells, and kinetic analyses demonstrate that Id2a is required for S-phase progression and/or the transition from S to M phase. Id2a-dependent defects in retinoblast proliferation lead to microphthalmia and to an absence of nearly all differentiated inner and outer nuclear layer cell types. Overexpression of id2a has the opposite effect on retinoblast cell cycle kinetics: id2a-overexpressing retinoblasts progress from S to M phase more rapidly and they undergo mitosis more frequently, which results in macrophthalmia. Mosaic analyses reveal that Id2a function in facilitating both cell cycle progression and neuronal differentiation in the retina is non-cell-autonomous, suggesting that Id2a functions upstream of the extrinsic pathways that regulate retinogenesis. |
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Authors:
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Rosa A Uribe; Jeffrey M Gross |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-10-13 |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 137 ISSN: 1477-9129 ISO Abbreviation: Development Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-27 Completed Date: 2010-11-10 Revised Date: 2011-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
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Languages: eng Pagination: 3763-74 Citation Subset: IM |
Affiliation:
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Section of Molecular Cell and Developmental Biology, Institute for Cell and Molecular Biology, The University of Texas at Austin, Austin, TX 78722, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle Kinetics Neurogenesis* Neuroglia / cytology, metabolism* Neurons / metabolism* Organ Size Retina / cytology, embryology*, metabolism Zebrafish / embryology*, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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F31-EY019239/EY/NEI NIH HHS; P40-RR012546/RR/NCRR NIH HHS; R01 EY018005-02/EY/NEI NIH HHS; R01 EY018005-03/EY/NEI NIH HHS; R01-EY18005/EY/NEI NIH HHS |
| Comments/Corrections | |
Erratum In:
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Development. 2011 Jan;138(1):179 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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