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Icariin: A Potential Promoting Compound for Cartilage Tissue Engineering.
MedLine Citation:
PMID:  22917745     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVE: To investigate whether icariin, which a widely used pharmacological constituent in traditional Chinese herbal medicine, can be a potential promoting compound for cartilage tissue engineering. DESIGN: Icariin was added into cell-hydrogel constructs derived from neonatal rabbit chondrocytes and collagen type I. The chondrogenic gene expressions and the synthesis of cartilage matrix of the seeded cells were detected by qRT-PCR and Biochemical assay. The effects of icariin-added cell-hydrogel constructs on the restoration of supercritical-sized osteochondral defects of adult rabbit were investigated by histological observation. The cell-hydrogel constructs without Icariin were set for controls. RESULTS: Icariin obviously up-regulate the expressions included aggrecan, sox9, and collagen type II of seeded chondrocytes form 99.7% to 248%. It increases the synthesis of glycosaminoglycan and collagen type II about fourfolds to fivefolds from week 1 to week 4, and accelerates the formation of chondroid tissue in the cell-hydrogel constructs. Even, it improves the restoration efficiency of super critical-sized osteochondral defects in adult rabbit model, and enhances the integration of new-formed cartilage with subchondral bone. CONCLUSIONS: Icariin can be a potential promoting compound for cartilage tissue engineering, and it can be a substitute for the use of some growth factors. The long history and extensive cases of safe use in China, Japan and Korea make it more attractive.
Authors:
Dongxiao Li; Tuan Yuan; Xuan Zhang; Yumei Xiao; Ru Wang; Yujiang Fan; Xingdong Zhang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-20
Journal Detail:
Title:  Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society     Volume:  -     ISSN:  1522-9653     ISO Abbreviation:  Osteoarthr. Cartil.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9305697     Medline TA:  Osteoarthritis Cartilage     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ltd.
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China; Sichuan Academy of Chinese Medicine Science, Chengdu, Sichuan, China.
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