| ISSa4-based differentiation of Streptococcus agalactiae strains and identification of multiple target sites for ISSa4 insertions. | |
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MedLine Citation:
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PMID: 14762005 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A collection of 113 epidemiologically unrelated Streptococcus agalactiae strains were studied (group B streptococcus; GBS): they belonged to different serotypes and were isolated from pregnant women in China and Russia. The insertion sequence ISSa4 was found in 21 of 113 strains (18,6%). All of the strains with ISSa4 belonged to serotypes II and II/c and were characterized by the presence of IS1381 and IS861 as well as the absence of IS1548 and GBSi1. All of the strains with ISSa4 possessed both bca and bac virulence genes coding for alpha and beta antigens, respectively. Among 21 ISSa4-positive strains, 13 different HindIII patterns (D1 to D13) hybridizing with an ISSa4 probe were found. One of them (D13) contained a single HindIII hybridization fragment 6.5 kb in size that was found to be specific for all ISSa4-positive GBS strains. Multiple target sites for insertions of ISSa4 were identified and included a putative pathogenicity island, "housekeeping" genes, and intergenic regions, as well as the genes for hypothetical proteins. No significant similarity was observed in the sequences of the target genes for ISSa4 insertions, in the relative location of the target genes on the chromosome, or the biological functions of the encoded proteins. The possible significance of ISSa4-based differentiation of the strains and the presence of possible "hot spots" for insertions of ISSa4 in GBS genome are discussed. |
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Authors:
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Alexander Dmitriev; Adong Shen; Xuzhuang Shen; Yonghong Yang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of bacteriology Volume: 186 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 2004 Feb |
Date Detail:
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Created Date: 2004-02-05 Completed Date: 2004-02-26 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 1106-9 Citation Subset: IM |
Affiliation:
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Institute of Experimental Medicine, Academy of Medical Sciences, Saint Petersburg 197376, Russia. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AY445916; AY449757; AY449758; AY449759; AY455996; AY455997; AY459524; AY461800; AY461801 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence DNA Transposable Elements* Female Gene Dosage Humans Molecular Sequence Data Polymorphism, Restriction Fragment Length Pregnancy Serotyping Streptococcus agalactiae / genetics* |
| Chemical | |
Reg. No./Substance:
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0/DNA Transposable Elements |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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