Document Detail

IS421, a new insertion sequence in Escherichia coli.
MedLine Citation:
PMID:  2542093     Owner:  NLM     Status:  MEDLINE    
The nucleotide sequence of a new insertion sequence (IS) in Escherichia coli, IS421, was determined. It is 1340 bp long and contains inverted repeats of 22 bp at its termini. It is flanked by 13 bp direct repeats apparently generated upon insertion. There are two ORFs longer than 200 bp in IS421. One can encode a polypeptide of 371 amino acids (aa) and the other, which is on the other strand, can encode a polypeptide of 102 aa. The C-terminal part of the 371 aa polypeptide shows some homology to that of transposases encoded in some other known IS elements. The copy number of IS421 in chromosomal DNA was 4 for E. coli K-12 and B, and 5 for E. coli C, as determined by the Southern hybridization of restriction fragments.
S Sato; Y Nakada; A Shiratsuchi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  249     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-06-28     Completed Date:  1989-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  21-6     Citation Subset:  IM    
Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
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MeSH Terms
Amino Acid Sequence
Base Sequence
DNA Probes
DNA Transposable Elements*
Escherichia coli / analysis,  genetics*
Molecular Sequence Data
Nucleic Acid Hybridization
Nucleotidyltransferases / genetics
Peptides / genetics
Reg. No./Substance:
0/DNA Probes; 0/DNA Transposable Elements; 0/Peptides; EC 2.7.7.-/Nucleotidyltransferases; EC 2.7.7.-/Transposases

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