Document Detail

IR super-resolution microspectroscopy and its application to single cells.
MedLine Citation:
PMID:  22356113     Owner:  NLM     Status:  MEDLINE    
For many years, spatial resolution is the most critical problem in IR microspectroscopy. This is because the spatial resolution of a conventional infrared microscope is restricted by the diffraction limit, which is almost the same as the wavelength of IR light, ranging from 2.5 to 25 μm. In the recent years, we have developed two novel types of far-field IR super-resolution microscopes using 2-color laser spectroscopies, those are transient fluorescence detected IR (TFD-IR) spectroscopy and vibrational sum-frequency generation (VSFG) spectroscopy. In these ways, because both transient fluorescence and VSFG signal have a wavelength in the visible region, the image is observed at the resolution of visible light, which is about 10 times smaller than that of IR light (that is, IR super-resolution). By using these techniques, we can map the specific IR absorption band with sub-micrometer spatial resolution, visualization of the molecular structure and reaction dynamics in a non-uniform environment such as a cell becomes a possibility. In the present reviews, we introduce our novel IR super-resolution microspectroscopy and its application to single cells in detail.
Makoto Sakai; Keiichi Inoue; Masaaki Fujii
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Current pharmaceutical biotechnology     Volume:  14     ISSN:  1873-4316     ISO Abbreviation:  Curr Pharm Biotechnol     Publication Date:  2013  
Date Detail:
Created Date:  2013-04-23     Completed Date:  2013-09-30     Revised Date:  2014-09-29    
Medline Journal Info:
Nlm Unique ID:  100960530     Medline TA:  Curr Pharm Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  159-66     Citation Subset:  IM    
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MeSH Terms
Cell Line, Tumor
Microscopy / instrumentation,  methods*
Plant Roots / cytology
Spectrophotometry, Infrared / instrumentation,  methods

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