Document Detail

IP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expression.
MedLine Citation:
PMID:  12415736     Owner:  NLM     Status:  MEDLINE    
Potassium depolarization of cultured muscle cells was employed to study cellular responses linked to calcium signaling. Events occurring after depolarization include i) A transient increase of the IP3 mass (2-10s); ii) A slow calcium transient (5 to 25s) that propagates as a low concentration wave along the myotube showing a distinct calcium transient at the level of cell nuclei. Due to the presence of IP3 receptors both in the SR (A-band region) and in the nuclear envelope, these two events appear to be related; iii) Phosphorylation of mitogen activated kinases (ERK 1/2) and of the transcription factor CREB (30 s-10 min), as well as expression of the early genes c-fos, c-jun and egr-1 mRNA (5-15 min). Several independent pieces of evidence, including results obtained using inhibitors specific for individual steps, allowed us to connect these in a sequential manner. As the same type of signaling cascade can be triggered by oxidants, neurotransmitters and hormones, the ensemble of results allows us to propose a general model to describe signaling events that link membrane stimulation to regulation of gene transcription in skeletal muscle cells.
Enrique Jaimovich; María Angélica Carrasco
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological research     Volume:  35     ISSN:  0716-9760     ISO Abbreviation:  Biol. Res.     Publication Date:  2002  
Date Detail:
Created Date:  2002-11-05     Completed Date:  2003-02-12     Revised Date:  2007-07-18    
Medline Journal Info:
Nlm Unique ID:  9308271     Medline TA:  Biol Res     Country:  Chile    
Other Details:
Languages:  eng     Pagination:  195-202     Citation Subset:  IM    
Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile Casilla 70005, Santiago 6530499, Chile.
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MeSH Terms
Calcium / metabolism
Calcium Channels / metabolism,  physiology*
Calcium Isotopes
Calcium Signaling / physiology*
Cells, Cultured
Cyclic AMP Response Element-Binding Protein / metabolism
Gene Expression Regulation*
Genes, Immediate-Early*
Inositol 1,4,5-Trisphosphate / metabolism*
Inositol 1,4,5-Trisphosphate Receptors
Muscle, Skeletal / cytology,  physiology*
RNA, Messenger / metabolism
Receptors, Cytoplasmic and Nuclear / metabolism,  physiology*
Ryanodine Receptor Calcium Release Channel / physiology
Transcription, Genetic
Reg. No./Substance:
0/Calcium Channels; 0/Calcium Isotopes; 0/Cyclic AMP Response Element-Binding Protein; 0/ITPR1 protein, human; 0/Inositol 1,4,5-Trisphosphate Receptors; 0/RNA, Messenger; 0/Receptors, Cytoplasmic and Nuclear; 0/Ryanodine Receptor Calcium Release Channel; 7440-70-2/Calcium; 85166-31-0/Inositol 1,4,5-Trisphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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