| IP-COSY, a totally in-phase and sensitive COSY experiment. | |
| | |
MedLine Citation:
|
PMID: 15706609 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The IP-COSY experiment presented in this paper gives an in-phase spectral presentation in both the F(1) and F(2) dimensions by a combined use of a constant evolution time (CT) in t(1) and a symmetrical refocusing period before t(2). Compared with DQF-COSY and CT-COSY, IP-COSY further alleviates the effect of signal reduction due to a small ratio p (= J/linewidth), showing (1) improved lineshape and cross-peak definition and (2) especially enhancement in signals of the peaks of small active J coupling constant and the peaks of broader linewidth. A new strategy was adopted to eliminate or reduce effectively artifactual peaks by adding a 0.1-0.2 ms variation to the time delays of the CT period used for each scan of the FID in IP-COSY and CT-COSY. (3)J(H,H) coupling constants of larger than 4 Hz in the fingerprint region of peptides can be directly derived from the separation of doublets. IP-COSY cross peaks are stronger than those in DQF-COSY by 4-20-fold for tested peptides and oligonucleotides (MW < 8 kDa) with acquisition and processing parameters used in the work, and they are easier to identify than those in CT-COSY. The overall improvement in IP-COSY should make the detection/autodetection of the COSY cross peaks and the measurements of the various coupling constants more easily achieved, providing valuable information for the structure elucidation of peptides/small proteins and oligonucleotides. |
| | |
Authors:
|
Youlin Xia; Glen Legge; Kyu-Yeon Jun; Yulan Qi; Hunjoong Lee; Xiaolian Gao |
Related Documents
:
|
17659539 - Correlations between mr and endothelial hyperplasia in low-grade gliomas. 16797939 - Decreased choline and creatine concentrations in centrum semiovale in patients with gen... 20803509 - Advanced magnetic resonance imaging in benign hereditary chorea: study of two familial ... 2083139 - Early and delayed imaging with 123i-imp spect in patients with ischemic cerebrovascular... 20373179 - Brain metabolic correlates of decision making in amnestic mild cognitive impairment. 18717729 - A 1h magnetic resonance spectroscopy study in patients with obstructive sleep apnea. 20519069 - Avoiding cavity surgery in penetrating torso trauma: the role of the computed tomograph... 6973379 - Radionuclide section scanning of the lungs in pulmonary embolism. 6779069 - Radiological techniques for small acoustic tumors--a re-evaluation. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Magnetic resonance in chemistry : MRC Volume: 43 ISSN: 0749-1581 ISO Abbreviation: Magn Reson Chem Publication Date: 2005 May |
Date Detail:
|
Created Date: 2005-04-12 Completed Date: 2005-08-29 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 9882600 Medline TA: Magn Reson Chem Country: England |
Other Details:
|
Languages: eng Pagination: 372-9 Citation Subset: IM |
Copyright Information:
|
Copyright (c) 2005 John Wiley & Sons, Ltd. |
Affiliation:
|
Department of Chemistry, University of Houston, Houston, Texas 77004-5003, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Nuclear Magnetic Resonance, Biomolecular
/
methods* Nucleic Acid Conformation Oligonucleotides / chemistry* Peptides / chemistry* Protein Conformation Proteins / chemistry* Time Factors |
| Grant Support | |
ID/Acronym/Agency:
|
GM49957/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Oligonucleotides; 0/Peptides; 0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Broadly distributed nucleophilic reactivity of proteins coordinated with specific ligand binding act...
Next Document: 1H, 13C and 19F NMR spectroscopy of polyfluorinated ureas. Correlations involving NMR chemical shift...