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IMPROVED Candida methylica FORMATE DEHYDROGENASE FERMENTATION THROUGH STATISTICAL OPTIMIZATION OF LOW-COST CULTURE MEDIA.
MedLine Citation:
PMID:  23030463     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
NAD(+)-dependent formate dehydrogenase (FDH, EC 1.2.1.2) is of use in the regeneration of NAD(P)H coenzymes, and therefore has strong potential for practical application in chemical and medical industries. A low-cost production of recombinant Escherichia coli (E. coli) containing FDH from Candida methylica (cmFDH) was optimized in molasses-based medium by using response surface methodology (RSM) based on central composite design (CCD). The beet molasses as a sole carbon source, (NH(4))(2)HPO(4) as a nitrogen and phosphorus source, KH(2)PO(4) as a buffer agent, and Mg(2)SO(4) · 7H(2)O as a magnesium and sulfur source were used as variables in the medium. The optimum medium composition was found to be 34.694 g L(-1) of reducing sugar (equivalent to molasses solution), 8.536 g L(-1) of (NH(4))(2)HPO(4), 3.073 g L(-1) of KH(2)PO(4), and 1.707 g L(-1) of Mg(2)SO(4) · 7H(2)O. Molasses-based culture medium increased the yield of cmFDH about three times compared to LB medium. The currently developed media has the potential to be used in industrial bioprocesses with low-cost production.
Authors:
Mustafa Kahraman; Emel Bıçakçı Ordu; Koray Yeşiladalı; Nevin Gül Karagüler; Candan Tamerler
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Preparative biochemistry & biotechnology     Volume:  42     ISSN:  1532-2297     ISO Abbreviation:  Prep. Biochem. Biotechnol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9607037     Medline TA:  Prep Biochem Biotechnol     Country:  England    
Other Details:
Languages:  eng     Pagination:  507-19     Citation Subset:  IM    
Affiliation:
a Faculty of Science and Letters, Department of Molecular Biology and Genetics , Istanbul Technical University , Istanbul , Turkey.
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