| IL-8 from local subcutaneous wounds regulates CD11b activation. | |
| | |
MedLine Citation:
|
PMID: 22229891 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The cellular and soluble mediators of a dermal inflammation can be studied by the skin chamber technique. The aim of the present study was to address the physiological effect of soluble mediators, released into the skin chamber, with special focus on neutrophil CD11b activation. Mediators released at the inflammatory site were studied by Milliplex and enzyme-linked immunosorbent assay (ELISA) and correlated to transmigration and CD11b activation in vivo and in vitro. Transmigration was studied by the skin chamber technique and by the transwell method and expression of the CBRM1/5 epitope on activated CD11b was analyzed by flow cytometry following in vivo and in vitro incubation with chamber fluid or recombinant interleukin-8 (IL-8). Leukocyte in vivo and in vitro transmigration both correlated to the concentrations of IL-1β, tumor necrosis factor alpha (TNFα) and IL-8 at p<0.05 (R>0.7). Furthermore, CD11b was activated, in terms of exposure of the activation epitope, on neutrophils after 30 minutes of in vitro incubation with chamber fluid and correlated solely to the concentration of IL-8, p<0.05 (R=0.72). In vitro incubation with recombinant IL-8 confirmed a concentration dependent expression of the activation epitope; however, induction of CBRM1/5 by recombinant IL-8 required a concentration which was significantly higher compared to that in chamber fluid. In addition, the CBRM1/5 epitope was analyzed on in vivo extravasated neutrophils which displayed a significantly higher expression compared to circulating neutrophils, p=0.04. We conclude that IL-8 is the major factor regulating the expression of CD11b activation epitope in neutrophils. |
| | |
Authors:
|
Joachim Lundahl; Stefan H Jacobson; Josefin M Paulsson |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-10 |
Journal Detail:
|
Title: Scandinavian journal of immunology Volume: - ISSN: 1365-3083 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0323767 Medline TA: Scand J Immunol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Scandinavian Journal of Immunology © 2012 Blackwell Publishing Ltd. |
Affiliation:
|
Department of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden. Department of Nephrology, Karolinska Institutet, Danderyd University Hospital, Stockholm, Sweden. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Oxidant stress, mitochondria, and cell death mechanisms in drug-induced liver injury: Lessons learne...
Next Document: Redox-dependent protein quality control in the ER: folding to degradation.