Document Detail

IL-6 and TNF-alpha expression in, and release from, contracting human skeletal muscle.
MedLine Citation:
PMID:  12388119     Owner:  NLM     Status:  MEDLINE    
The aim of the present study was to examine whether IL-6 and TNF-alpha are expressed in, and released from, human skeletal muscle during exercise. We hypothesized that the skeletal muscle will release IL-6, but not TNF-alpha, during exercise because of previous observations that TNF-alpha negatively affects glucose uptake in skeletal muscle. Six healthy, male subjects performed 180 min of two-legged knee-extensor exercise. Muscle samples were obtained from the vastus lateralis of one limb. In addition, blood samples were obtained from a femoral artery and vein. Plasma was analyzed for IL-6 and TNF-alpha. We detected both IL-6 and TNF-alpha mRNA in resting muscle samples, and whereas IL-6 increased (P < 0.05) approximately 100-fold throughout exercise, no significant increase in TNF-alpha mRNA was observed. Arterial plasma TNF-alpha did not increase during exercise. Furthermore, there was no net release of TNF-alpha either before or during exercise. In contrast, IL-6 increased throughout exercise in arterial plasma, and a net IL-6 release from the contracting limb was observed after 120 min of exercise (P < 0.05).
Adam Steensberg; Charlotte Keller; Rebecca L Starkie; Takuya Osada; Mark A Febbraio; Bente Klarlund Pedersen
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-08-20
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  283     ISSN:  0193-1849     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-08     Completed Date:  2002-12-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E1272-8     Citation Subset:  IM    
Copenhagen Muscle Research Centre and Department of Infectious Diseases, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen, Denmark.
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MeSH Terms
Blood Glucose / analysis
Exercise Test
Fatty Acids, Nonesterified / blood
Femoral Artery / physiology
Femoral Vein / physiology
Glycogen / analysis,  metabolism
Interleukin-6 / genetics,  metabolism*,  secretion
Leg / blood supply,  physiology
Muscle Contraction / physiology*
Muscle, Skeletal / chemistry,  metabolism*,  secretion
Physical Exertion / physiology
RNA, Messenger / analysis,  metabolism
Reference Values
Regional Blood Flow
Tumor Necrosis Factor-alpha / genetics,  metabolism*,  secretion
Reg. No./Substance:
0/Blood Glucose; 0/Fatty Acids, Nonesterified; 0/Interleukin-6; 0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha; 9005-79-2/Glycogen

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