| IL-4-induced AID expression and its relevance to IgA class switch recombination. | |
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MedLine Citation:
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PMID: 17645870 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Activation-induced cytidine deaminase (AID) is an inducible gene that plays a critical role in Ig class switch recombination and somatic hypermutation in B cells. We explored the mechanisms by which IL-4 induces AID expression in mouse B cells. IL-4 increased AID expression and over-expression of Stat6 further augmented IL-4-induced promoter activity. The involvement of Stat6 in the promoter activity was confirmed using ChIP assays and site-directed mutagenesis. Treatment with H89, a PKA inhibitor, markedly decreased IL-4-induced AID expression, and over-expression of CREB enhanced it. These results indicate that Stat6 and PKA/CREB are involved in IL-4-induced AID expression. The relevance of these signal transducing molecules was verified using the TGFbeta1-induced IgA isotype switching model. Our results indicate that IL-4, through Stat6 and PKA/CREB, induces AID expression leading to Ig isotype switching event. |
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Authors:
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Ran Ju Kim; Hyun-A Kim; Jae-Bong Park; Seok-Rae Park; Seong-Hyun Jeon; Goo-Young Seo; Dong-Wan Seo; Su Ryeon Seo; Gie-Taek Chun; Nam-Soo Kim; Se-Won Yie; Woo-Hyeon Byeon; Pyeung-Hyeun Kim |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-07-16 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 361 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2007 Sep |
Date Detail:
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Created Date: 2007-08-07 Completed Date: 2007-09-28 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 398-403 Citation Subset: IM |
Affiliation:
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Department of Molecular Bioscience, School of Bioscience and Biotechnology, Kangwon National University, Chunchon 200-701, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line, Tumor Cyclic AMP Response Element-Binding Protein / metabolism Cyclic AMP-Dependent Protein Kinases / metabolism Cytidine Deaminase / biosynthesis*, genetics Enzyme Induction / drug effects Immunoglobulin A / genetics* Immunoglobulin Class Switching / drug effects, genetics* Interleukin-4 / pharmacology* Mice Models, Immunological Promoter Regions, Genetic / genetics Recombination, Genetic / drug effects, genetics* STAT6 Transcription Factor / metabolism Transforming Growth Factor beta1 / pharmacology p38 Mitogen-Activated Protein Kinases / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cyclic AMP Response Element-Binding Protein; 0/Immunoglobulin A; 0/STAT6 Transcription Factor; 0/Transforming Growth Factor beta1; 207137-56-2/Interleukin-4; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases; EC 3.5.4.-/AICDA (activation-induced cytidine deaminase); EC 3.5.4.5/Cytidine Deaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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