| IL-27 Enhances LPS-Induced Proinflammatory Cytokine Production via Upregulation of TLR4 Expression and Signaling in Human Monocytes. | |
| | |
MedLine Citation:
|
PMID: 22156348 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
IL-27, which is produced by activated APCs, bridges innate and adaptive immunity by regulating the development of Th cells. Recent evidence supports a role for IL-27 in the activation of monocytic cells in terms of inflammatory responses. Indeed, proinflammatory and anti-inflammatory activities are attributed to IL-27, and IL-27 production itself is modulated by inflammatory agents such as LPS. IL-27 primes LPS responses in monocytes; however, the molecular mechanism behind this phenomenon is not understood. In this study, we demonstrate that IL-27 priming results in enhanced LPS-induced IL-6, TNF-α, MIP-1α, and MIP-1β expression in human primary monocytes. To elucidate the molecular mechanisms responsible for IL-27 priming, we measured levels of CD14 and TLR4 required for LPS binding. We determined that IL-27 upregulates TLR4 in a STAT3- and NF-κB-dependent manner. Immunofluorescence microscopy revealed enhanced membrane expression of TLR4 and more distinct colocalization of CD14 and TLR4 upon IL-27 priming. Furthermore, IL-27 priming enhanced LPS-induced activation of NF-κB family members. To our knowledge, this study is the first to show a role for IL-27 in regulating TLR4 expression and function. This work is significant as it reveals new mechanisms by which IL-27 can enhance proinflammatory responses that can occur during bacterial infections. |
| | |
Authors:
|
Christina Guzzo; Amit Ayer; Sameh Basta; Bruce W Banfield; Katrina Gee |
Related Documents
:
|
18550788 - Recruitment of the inflammatory subset of monocytes to sites of ischemia induces angiog... 397718 - In vitro influence of endotoxin on human mononuclear phagocyte structure and function. ... 18641108 - Increased expression of cd16, cd69, and very late antigen-1 on blood monocytes in activ... 10867598 - Soot-exposed mononuclear cells increase inflammatory cytokine mrna expression and prote... 7495758 - The primary alloresponse of human cd4+ t cells is dependent on b7 (cd80), augmented by ... 10575668 - Asthma, oxidant stress, and diet. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-7 |
Journal Detail:
|
Title: Journal of immunology (Baltimore, Md. : 1950) Volume: - ISSN: 1550-6606 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-13 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2985117R Medline TA: J Immunol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Trogocytosis Is a Gateway to Characterize Functional Diversity in Melanoma-Specific CD8+ T Cell Clon...
Next Document: Type I IFN-Dependent T Cell Activation Is Mediated by IFN-Dependent Dendritic Cell OX40 Ligand Expre...