Document Detail


IL-2 gene transfer in immunotherapy of cancer: local administration of IL-2-activated lymphocytes and X63-m-IL-2 cells constitutively producing IL-2 inhibits growth of plasmacytomas in syngeneic mice.
MedLine Citation:
PMID:  1758466     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Peritumoral administration of X63-m-IL-2 cells, transformed by IL-2 cDNA and constitutively producing large quantities of IL-2, was effective in mediating regression of X63-Ag8.653 plasmacytomas transplanted in syngeneic mice. Injection of killer cells with LAK activity substantially enhanced the tumor-inhibitory effect of IL-2-producing cells. In vitro activation of murine bone marrow and spleen cells with IL-2 produced by the X63-m-IL-2 cell line resulted in generation of lymphocytes with oncolytic activity against X63-Ag8.653 plasmacytomas and a variety of other target cell lines. The cytolytic activity of adherent IL-2-activated killer cells (A-LAK) was substantially higher than that of a nonadherent subset. Bone marrow and spleen cells from both healthy control and tumor-bearing mice contained LAK precursors, but the cytolytic activity derived from tumor-bearing mice was lower than that from healthy controls.
Authors:
J Bubeník; E Lotzová; M Indrová; J Simová; T Jandlová; C A Savary
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Natural immunity and cell growth regulation     Volume:  10     ISSN:  0254-7600     ISO Abbreviation:  Nat. Immun. Cell Growth Regul.     Publication Date:  1991  
Date Detail:
Created Date:  1992-02-04     Completed Date:  1992-02-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8407979     Medline TA:  Nat Immun Cell Growth Regul     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  247-55     Citation Subset:  IM    
Affiliation:
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line, Transformed / immunology
Cytotoxicity, Immunologic
Immunotherapy, Adoptive*
Interleukin-2 / biosynthesis,  genetics,  therapeutic use*
Killer Cells, Lymphokine-Activated / immunology*
Lymphocyte Activation
Lymphocytes, Tumor-Infiltrating / immunology
Mice
Mice, Inbred BALB C
Neoplasm Transplantation
Plasmacytoma / therapy*
Transfection
Transplantation, Isogeneic
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
CA 39632/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Interleukin-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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