| IL-1alpha secreted by colon cancer cells enhances angiogenesis: the relationship between IL-1alpha release and tumor cells' potential for liver metastasis. | |
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MedLine Citation:
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PMID: 19204921 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND OBJECTIVES: Interleukin (IL)-1alpha plays an important role in colon cancer progression and angiogenesis. We here asked whether IL-1alpha derived from cancer cells modulates vascular endothelial cell growth, migration and tubule formation. METHODS: The existence of IL-1alpha mRNA and protein in colon cancer cell lines (WiDr, HT-29, Caco-2, COLO 320) were investigated with RT-PCR and ELISA. Proliferation and invasion were investigated by MTS assay and Matrigel-double chamber assay. To answer our main question, we performed angiogenesis assay used an in vitro model consisting of co-cultivated tumor cells and stromal cells. RESULTS: IL-1alpha mRNA and protein were detected in highly metastatic colon cancer cells (WiDr and HT-29). Recombinant IL-1alpha significantly enhanced growth and invasiveness of human umbilical vein endothelial cells (HUVEC) (P < 0.01). Moreover, HUVEC growth and migration were significantly enhanced by WiDr compared to control (without co-culture) or Caco-2 (P < 0.05). Exogenous rIL-1alpha significantly enhanced HUVEC tube-like formation in a dose-dependent manner (P < 0.01) in a HUVEC/fibroblast co-cultivation system. Moreover, WiDr significantly enhanced HUVEC tubule formation compared with control or Caco-2 (P < 0.01). CONCLUSION: Based on these findings, we conclude that colon cancer cell-derived IL-1alpha up-regulates angiogenesis by modulating stromal cells within the tumor cells' microenvironment. |
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Authors:
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Yoichi Matsuo; Hirozumi Sawai; Jiachi Ma; Donghui Xu; Nobuo Ochi; Akira Yasuda; Hiroki Takahashi; Hitoshi Funahashi; Hiromitsu Takeyama |
Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of surgical oncology Volume: 99 ISSN: 1096-9098 ISO Abbreviation: J Surg Oncol Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-21 Completed Date: 2009-05-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0222643 Medline TA: J Surg Oncol Country: United States |
Other Details:
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Languages: eng Pagination: 361-7 Citation Subset: IM |
Affiliation:
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Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya 4678601, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Caco-2 Cells Cell Line, Tumor Cell Proliferation Colonic Neoplasms / blood supply*, pathology, secretion* Enzyme-Linked Immunosorbent Assay Humans Interleukin-1alpha / genetics, secretion* Liver Neoplasms / blood supply*, secondary* Neoplasm Invasiveness Neovascularization, Pathologic / metabolism* RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-1alpha; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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