Document Detail

IL-1alpha secreted by colon cancer cells enhances angiogenesis: the relationship between IL-1alpha release and tumor cells' potential for liver metastasis.
MedLine Citation:
PMID:  19204921     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND OBJECTIVES: Interleukin (IL)-1alpha plays an important role in colon cancer progression and angiogenesis. We here asked whether IL-1alpha derived from cancer cells modulates vascular endothelial cell growth, migration and tubule formation. METHODS: The existence of IL-1alpha mRNA and protein in colon cancer cell lines (WiDr, HT-29, Caco-2, COLO 320) were investigated with RT-PCR and ELISA. Proliferation and invasion were investigated by MTS assay and Matrigel-double chamber assay. To answer our main question, we performed angiogenesis assay used an in vitro model consisting of co-cultivated tumor cells and stromal cells. RESULTS: IL-1alpha mRNA and protein were detected in highly metastatic colon cancer cells (WiDr and HT-29). Recombinant IL-1alpha significantly enhanced growth and invasiveness of human umbilical vein endothelial cells (HUVEC) (P < 0.01). Moreover, HUVEC growth and migration were significantly enhanced by WiDr compared to control (without co-culture) or Caco-2 (P < 0.05). Exogenous rIL-1alpha significantly enhanced HUVEC tube-like formation in a dose-dependent manner (P < 0.01) in a HUVEC/fibroblast co-cultivation system. Moreover, WiDr significantly enhanced HUVEC tubule formation compared with control or Caco-2 (P < 0.01). CONCLUSION: Based on these findings, we conclude that colon cancer cell-derived IL-1alpha up-regulates angiogenesis by modulating stromal cells within the tumor cells' microenvironment.
Yoichi Matsuo; Hirozumi Sawai; Jiachi Ma; Donghui Xu; Nobuo Ochi; Akira Yasuda; Hiroki Takahashi; Hitoshi Funahashi; Hiromitsu Takeyama
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of surgical oncology     Volume:  99     ISSN:  1096-9098     ISO Abbreviation:  J Surg Oncol     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-21     Completed Date:  2009-05-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0222643     Medline TA:  J Surg Oncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  361-7     Citation Subset:  IM    
Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya 4678601, Japan.
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MeSH Terms
Caco-2 Cells
Cell Line, Tumor
Cell Proliferation
Colonic Neoplasms / blood supply*,  pathology,  secretion*
Enzyme-Linked Immunosorbent Assay
Interleukin-1alpha / genetics,  secretion*
Liver Neoplasms / blood supply*,  secondary*
Neoplasm Invasiveness
Neovascularization, Pathologic / metabolism*
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Interleukin-1alpha; 0/RNA, Messenger

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