Document Detail


IL-18 enhances the migration ability of murine melanoma cells through the generation of ROI and the MAPK pathway.
MedLine Citation:
PMID:  17014914     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interleukin-18 (IL-18) has multiple effects on various cells that are involved in immune escape of murine melanoma cells and in the inflammatory responses. This study investigated the effect of IL-18 on the ability of murine melanoma cells to migrate by using B16F10 cells and the IL-18 antisense transfectants of B16F10 cells (the B16F10/IL-18 antisense transfectant). The B16F10 cells were more able to migrate than were the B16F10/IL-18 antisense transfectants. An exogenous IL-18 treatment improved the ability of the B16F10/IL-18 antisense transfectant cells to migrate, indicating that IL-18 enhanced the migration ability of melanoma cells. To determine the signaling mechanisms involved in IL-18-enhanced migration, we measured the ROI levels. It was found that the ROI levels were increased by IL-18, and an antioxidant, N-acetyl-l-cystein (NAC), blocked the effect of IL-18 on migration, suggesting the involvement of ROI in the signal transduction of IL-18-enhanced cell migration. IL-18-enhanced cell migration was also reduced by PD98059. In addition, the level of ERK1/2 phosphorylation was markedly increased by treating with exogenous IL-18 at 20 min. These results suggest that IL-18 enhances the ability of melanoma cells to migrate via the generation of ROI and the MAPK pathway.
Authors:
Min Kyung Jung; Hyun Keun Song; Kyung-Eun Kim; Dae Young Hur; Taesung Kim; Saic Bang; Hyunjeong Park; Dae Ho Cho
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-11
Journal Detail:
Title:  Immunology letters     Volume:  107     ISSN:  0165-2478     ISO Abbreviation:  Immunol. Lett.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-27     Completed Date:  2007-02-05     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  125-30     Citation Subset:  IM    
Affiliation:
Department of Life Science, Sookmyung Women's University, Chungpa-Dong 2-Ka, Yongsan-ku, Seoul 140-742, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Acetylcysteine / pharmacology
Animals
Cell Movement* / drug effects
Flavonoids / pharmacology
Free Radical Scavengers / pharmacology
Interleukin-18 / genetics,  pharmacology,  physiology*
Melanoma, Experimental / immunology,  pathology*
Mice
Mitogen-Activated Protein Kinase 1 / antagonists & inhibitors,  metabolism
Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors,  metabolism
Mitogen-Activated Protein Kinases / antagonists & inhibitors,  metabolism*
Phosphorylation
Protein Kinase Inhibitors / pharmacology
Reactive Oxygen Species / analysis,  antagonists & inhibitors,  metabolism*
Transfection
Chemical
Reg. No./Substance:
0/2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; 0/Flavonoids; 0/Free Radical Scavengers; 0/Interleukin-18; 0/Protein Kinase Inhibitors; 0/Reactive Oxygen Species; 616-91-1/Acetylcysteine; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3; EC 2.7.11.24/Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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