| Interleukin-15 receptor α expression in inflammatory bowel disease patients before and after normalization of inflammation with infliximab. | |
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MedLine Citation:
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PMID: 23039249 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Interleukin-15 (IL-15) is a pro-inflammatory cytokine thought to contribute to the inflammation in inflammatory bowel diseases (IBD). The specific receptor chain IL-15Rα can be expressed as a transmembranous signalling receptor, or can be cleaved by a disintegrin and metalloprotease domain 17 (ADAM17) into a neutralizing, soluble receptor (sIL-15Rα). The aim of this study is to evaluate the expression of IL-15Rα in ulcerative colitis (UC) and Crohn's disease (CD) patients before and after infliximab (IFX) therapy. Gene expression of IL-15Rα, IL-15 and ADAM17 was measured at the mRNA level by quantitative reverse transcription-PCR in mucosal biopsies harvested before and after first IFX therapy. Concentrations of sIL-15Rα were measured in sera of patients by ELISA and IL-15Rα protein was localized in the gut by immunohistochemistry and immunofluorescence. Mucosal expression of IL-15Rα is increased in UC and CD patients compared with controls and it remains elevated after IFX therapy in both responder and non-responder patients. The concentration of sIL-15Rα in serum is also increased in UC patients when compared with controls and does not differ between responders and non-responders either before or after IFX. CD patients have levels of sIL-15Rα comparable to healthy controls before and after therapy. In mucosal tissues, IL-15Rα(+) cells closely resemble activated memory B cells with a pre-plasmablastic phenotype. To conclude, IBD patients have an increased expression of IL-15Rα mRNA in the mucosa. Expression is localized in B cells, suggesting that IL-15 regulates B-cell functions during bowel inflammation. No change in release of sIL-15Rα is observed in patients treated with IFX. |
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Authors:
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Clémentine Perrier; Ingrid Arijs; Dominiek Staelens; Christine Breynaert; Isabelle Cleynen; Kris Covens; Marc Ferrante; Gert Van Assche; Séverine Vermeire; Gert de Hertogh; Frans Schuit; Paul Rutgeerts; Jan L Ceuppens |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Immunology Volume: 138 ISSN: 1365-2567 ISO Abbreviation: Immunology Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2012-12-17 Completed Date: 2013-03-27 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0374672 Medline TA: Immunology Country: England |
Other Details:
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Languages: eng Pagination: 47-56 Citation Subset: IM |
Copyright Information:
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© 2012 Blackwell Publishing Ltd. |
Affiliation:
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Department of Gastroenterology, Translational Research Centre for Gastrointestinal Disorders (TARGID), KU Leuven, Leuven, Belgium. Clementine.Perrier@med.kuleuven.be |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Anti-Inflammatory Agents, Non-Steroidal / pharmacology*, therapeutic use Antibodies, Monoclonal / pharmacology*, therapeutic use* Colitis, Ulcerative / drug therapy*, immunology Crohn Disease / drug therapy*, immunology Enzyme-Linked Immunosorbent Assay Female Fluorescent Antibody Technique Humans Immunohistochemistry Inflammation / drug therapy Interleukin-15 Receptor alpha Subunit / biosynthesis*, genetics, immunology* Male Middle Aged RNA, Messenger / genetics Reverse Transcriptase Polymerase Chain Reaction Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents, Non-Steroidal; 0/Antibodies, Monoclonal; 0/Interleukin-15 Receptor alpha Subunit; 0/RNA, Messenger; 0/infliximab |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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