Document Detail

IL-13Ralpha2 is a glioma-restricted receptor for interleukin-13.
MedLine Citation:
PMID:  12192597     Owner:  NLM     Status:  MEDLINE    
We have found that binding sites for interleukin-13 (IL-13) are overexpressed in a vast majority of high-grade astrocytomas (HGAs). These binding sites for IL-13 are distinct from the physiological receptor in that it does not bind IL-4. We also demonstrated that IL-13 receptor alpha 2 protein chain (IL-13Ralpha2), an IL-4-independent receptor for IL-13, is abundant among HGAs, but not in normal organs. To examine if IL-13Ralpha2 is the tumor-associated site for IL-13, we stably transfected normal Chinese hamster ovary (CHO) cells and glioma G-26 cells to express either human (h) or murine (m) IL-13Ralpha2. CHO-hIL-13Ralpha2(+) cells and G-26-h/mIL-13Ralpha2(+) cells, and not CHO and G-26 parental or mock-transfected cells, specifically bound IL-13 in an IL-4-independent manner. The IL-13Ralpha2(+) cells also became highly susceptible to the killing by an IL-13-based cytotoxic fusion protein. In loss of function studies, a HGA cell line, SNB-19, was transfected with antisense (as) hIL-13Ralpha2. as-SNB-19-hIL-13Ralpha2(+) cells lost their natural affinity towards IL-13 and became resistant to IL-13-based cytotoxins. The fact, that IL-13Ralpha2-positive cells bind IL-13 independent of IL-4, become susceptible to IL-13 cytotoxins, and cells deprived of IL-13Ralpha2 receptor lose these features, demonstrates that IL-13Ralpha2 is the brain tumor-associated receptor for IL-13.
Akiva Mintz; Denise M Gibo; Becky Slagle-Webb; Neil D Christensen; Waldemar Debinski
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neoplasia (New York, N.Y.)     Volume:  4     ISSN:  1522-8002     ISO Abbreviation:  Neoplasia     Publication Date:    2002 Sep-Oct
Date Detail:
Created Date:  2002-08-22     Completed Date:  2003-02-20     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  100886622     Medline TA:  Neoplasia     Country:  United States    
Other Details:
Languages:  eng     Pagination:  388-99     Citation Subset:  IM    
Section of Neurosurgery/H110, Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033-0850, USA.
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MeSH Terms
Binding Sites
Blotting, Northern
Brain Neoplasms / metabolism*,  pathology
CHO Cells / metabolism
DNA Primers / chemistry
DNA, Antisense / pharmacology
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Glioma / metabolism*,  pathology
Interleukin-13 / metabolism*
Interleukin-13 Receptor alpha1 Subunit
Interleukin-4 / metabolism
Polymerase Chain Reaction
Protein Binding
RNA / metabolism
Receptors, Interleukin / genetics,  metabolism*
Receptors, Interleukin-13
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/DNA Primers; 0/DNA, Antisense; 0/IL13RA1 protein, human; 0/Interleukin-13; 0/Interleukin-13 Receptor alpha1 Subunit; 0/Receptors, Interleukin; 0/Receptors, Interleukin-13; 207137-56-2/Interleukin-4; 63231-63-0/RNA

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